RegulonDB

TRANSCRIPTION FACTOR:

Name:

CRP	Weight Matrix Tractordb tool

Connectivity class

Global Regulator

Sensing Class

Using internal synthesized signals

Synonym(s):

Cap, Csm, DNA-binding transcriptional dual regulator CRP, GurB

Gene name(s):

crp

Functional conformation(s):

CRP , CRP-cyclic-AMP

Coregulator(s):

AaeR, AcrR, AdiY, AgaR, AraC, ArcA, ArgP, ArgR, AscG, BasR, BolA, BtsR, CRP, CRP-Sxy, CaiF, ChbR, ComR, CpxR, Cra, CreB, CsgD, CspA, CsqR, CusR, CytR, DcuR, DeoR, DgoR, DksA-ppGpp, DnaA, DpiA, DsdC, EbgR, EnvY, EvgA, ExuR, FNR, FadR, FeaR, FhlA, Fis, FlhDC, FliZ, FrlR, FucR, Fur, GadE, GadE-RcsB, GadW, GadX, GalR, GalS, GatR, GcvA, GlaR, GlcC, GlpR, GlrR, GntR, GutM, H-NS, HU, HdfR, HipB, HprR, HyfR, HypT, IHF, IclR, IdnR, LacI, LeuO, LexA, LrhA, Lrp, LsrR, MalI, MalT, MarA, MarR, MatA, McbR, MelR, MetJ, MhpR, Mlc, MlrA, ModE, MprA, MqsA, MurR, Nac, NadR, NagC, NanR, NarL, NarP, NrdR, NsrR, NtrC, OmpR, OxyR, PaaX, PdhR, PhoB, PhoP, PlaR, PrpR, PurR, PuuR, QseB, RbsR, RcdA, RcsAB, RcsB, RcsB-BglJ, RhaR, RhaS, Rob, RspR, RstA, SdiA, SlyA, SoxR, SoxS, SrlR, StpA, TdcA, TdcR, TorR, TreR, UhpA, UidR, UlaR, UvrY, UxuR, XylR, YdeO, YjjQ, ZraR, ppGpp

Summary:

TF Summary ...[more]

TRANSCRIPTION FACTOR:

Info

Name:

RhaS	Weight Matrix Tractordb tool

Connectivity class

Local Regulator

Sensing Class

External sensing using transported metabolites

Synonym(s):

DNA-binding transcriptional activator RhaS

Gene name(s):

rhaS

Functional conformation(s):

RhaS-α-L-rhamnopyranose , RhaS , RhaS-L-rhamnose

Coregulator(s):

ArcA, CRP, RhaR, RhaS

Summary:

TF Summary ...[more]

REGULATION EXERTED BY CRP,RhaS

Info M3D | Colombos

KNOWN BINDING SITES (The central relative position is relative to the promoter +1)
CRP activator RhaS activator
Transcription Factor		Regulated		Binding Sites				Evidence	References
Functional conformation	Function	Promoter	Gene(s)	LeftPos	RigthPos	Central Rel-Pos	Sequence
CRP-cyclic-AMP	activator	rhaBp	rhaD , rhaA , rhaB	4097584	4097605	-122.5	tcaggaaatgCGGTGAGCATCACATCACCACAattcagcaaa	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[1]
RhaS-L-rhamnose	activator	rhaBp	rhaD , rhaA , rhaB	4097504	4097520	-40.0	ccattttcctGTCAGTAACGAGAAGGTcgcgaattca	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3] [4]
RhaS-L-rhamnose	activator	rhaBp	rhaD , rhaA , rhaB	4097537	4097553	-73.0	catcacgttcATCTTTCCCTGGTTGCCaatggcccat	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[5] [6] [2] [3] [1] [7] [4]
CRP-cyclic-AMP	activator	rhaBp	rhaD , rhaA , rhaB	4097554	4097575	-92.5	caattcagcaAATTGTGAACATCATCACGTTCatctttccct	[EXP-IEP-GENE-EXPRESSION-ANALYSIS]	[2] [3]

Evidence:	[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis
Reference(s):	[1] Holcroft CC., et al., 2000 [2] Egan SM., et al., 1993 [3] Holcroft CC., et al., 2000 [4] Wickstrum JR., et al., 2007 [5] Bhende PM., et al., 1999 [6] Egan SM., et al., 1994 [7] Wickstrum JR., et al., 2004

Reference(s)
[1] Holcroft CC., Egan SM., 2000, Interdependence of activation at rhaSR by cyclic AMP receptor protein, the RNA polymerase alpha subunit C-terminal domain, and rhaR., J Bacteriol 182(23):6774-82
[2] Egan SM., Schleif RF., 1993, A regulatory cascade in the induction of rhaBAD., J Mol Biol 234(1):87-98
[3] Holcroft CC., Egan SM., 2000, Roles of cyclic AMP receptor protein and the carboxyl-terminal domain of the alpha subunit in transcription activation of the Escherichia coli rhaBAD operon., J Bacteriol 182(12):3529-35
[4] Wickstrum JR., Skredenske JM., Kolin A., Jin DJ., Fang J., Egan SM., 2007, Transcription activation by the DNA-binding domain of the AraC family protein RhaS in the absence of its effector-binding domain., J Bacteriol 189(14):4984-93
[5] Bhende PM., Egan SM., 1999, Amino acid-DNA contacts by RhaS: an AraC family transcription activator., J Bacteriol 181(17):5185-92
[6] Egan SM., Schleif RF., 1994, DNA-dependent renaturation of an insoluble DNA binding protein. Identification of the RhaS binding site at rhaBAD., J Mol Biol 243(5):821-9
[7] Wickstrum JR., Egan SM., 2004, Amino acid contacts between sigma 70 domain 4 and the transcription activators RhaS and RhaR., J Bacteriol 186(18):6277-85