RegulonDB

Gene
Name:	dcuB
Synonym(s):	ECK4116, EG10006, b4123, genF
Genome position(nucleotides):	4347404 <-- 4348744 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.27
External database links:

ASAP:	ABE-0013503
CGSC:	34479
ECHOBASE:	EB0006
ECOLIHUB:	dcuB
OU-MICROARRAY:	b4123
STRING:	511145.b4123
COLOMBOS:	dcuB

Product

Name:

anaerobic C4-dicarboxylate transporter DcuB

Synonym(s):

DcuB, GenF

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

47.935

Isoelectric point:

7.878

Motif(s):

Type	Positions	Sequence
	6 -> 376	QLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREYVYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMFMLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDP
	21 -> 39	GGIALGLLGGIGLVILVFV
	56 -> 73	IAVVAASATLQASGGLDV
	367 -> 384	PVALAIGVDPAYIVASAP
	119 -> 119	D

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0005515 - protein binding GO:0015556 - C4-dicarboxylate transmembrane transporter activity GO:0005469 - succinate:fumarate antiporter activity
	biological_process	GO:0009061 - anaerobic respiration GO:0015740 - C4-dicarboxylate transport GO:0015741 - fumarate transport GO:0071422 - succinate transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Engel P., et al., 1992
[2] Scheu PD., et al., 2010

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

dcuB-fumB

Operon arrangement:

Transcription unit	Promoter
fumB	fumBp
dcuB-fumB	dcuBp1
dcuB-fumB	dcuBp2

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, FNR, DcuR
Repressed by:	NarL

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_4923 (cluster)	4347981	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_4924	4348786	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_4925	4348841	reverse	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Engel P., Kramer R., Unden G., 1992, Anaerobic fumarate transport in Escherichia coli by an fnr-dependent dicarboxylate uptake system which is different from the aerobic dicarboxylate uptake system., J Bacteriol 174(17):5533-9
[2] Scheu PD., Kim OB., Griesinger C., Unden G., 2010, Sensing by the membrane-bound sensor kinase DcuS: exogenous versus endogenous sensing of C(4)-dicarboxylates in bacteria., Future Microbiol 5(9):1383-402
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.