RegulonDB RegulonDB 11.1: Gene Form
   

aroF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tyrA aroF yfiL TyrR TyrR TyrR SoxR yfiRp8 yfiRp8 yfiRp7 yfiRp7 yfiLp10 yfiLp10 yfiLp8 yfiLp8 yfiLp9 yfiLp9 aroFp aroFp TSS_2942 TSS_2942 TSS_2941 TSS_2941

Gene      
Name: aroF    Texpresso search in the literature
Synonym(s): ECK2598, EG10078, b2601
Genome position(nucleotides): 2740080 <-- 2741150
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.82
External database links:  
ASAP:
 ABE-0008549
CGSC:
 1003
ECHOBASE:
 EB0076
ECOLIHUB:
 aroF
OU-MICROARRAY:
 b2601
STRING:
 511145.b2601
COLOMBOS: aroF


Product      
Name: 3-deoxy-7-phosphoheptulonate synthase, Tyr-sensitive
Synonym(s): 2-dehydro-3-deoxyphosphoheptonate aldolase, AroF
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 38.804
Isoelectric point: 5.41
Motif(s):
 
Type Positions Sequence Comment
39 -> 340 DSRKSISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALL

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.20 - chorismate
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0003849 - 3-deoxy-7-phosphoheptulonate synthase activity
GO:0042802 - identical protein binding
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009058 - biosynthetic process
GO:0009073 - aromatic amino acid family biosynthetic process
GO:0009423 - chorismate biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Ray JM., et al., 1988
External database links:  
ALPHAFOLD:
 P00888
DIP:
 DIP-9154N
ECOCYC:
 AROF-MONOMER
ECOLIWIKI:
 b2601
INTERPRO:
 IPR006219
INTERPRO:
 IPR006218
INTERPRO:
 IPR013785
MODBASE:
 P00888
PANTHER:
 PTHR21225
PDB:
 6AGL
PDB:
 6AGM
PFAM:
 PF00793
PRIDE:
 P00888
PRODB:
 PRO_000022147
REFSEQ:
 NP_417092
SMR:
 P00888
SWISSMODEL:
 P00888
UNIPROT:
 P00888


Operon      
Name: aroF-tyrA         
Operon arrangement:
Transcription unit        Promoter
aroF-tyrA


Transcriptional Regulation      
Display Regulation             
Activated by: SoxR
Repressed by: TyrR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2941 2739800 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2942 2740969 reverse nd [RS-EPT-CBR] [2]
  promoter yfiLp9 2741315 forward nd [COMP-AINF] [3]
  promoter yfiLp8 2741347 forward nd [COMP-AINF] [3]
  promoter yfiLp10 2741355 forward nd [COMP-AINF] [3]
  promoter yfiRp7 2741677 forward nd [COMP-AINF] [3]
  promoter yfiRp8 2741702 forward nd [COMP-AINF] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Ray JM., Yanofsky C., Bauerle R., 1988, Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli., J Bacteriol 170(12):5500-6

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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