RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

atpI gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	atpI
Synonym(s):	ECK3732, EG10106, b3739, uncI
Genome position(nucleotides):	3922060 <-- 3922440 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.23
External database links:

ASAP:	ABE-0012224
CGSC:	18511
ECHOBASE:	EB0104
OU-MICROARRAY:	b3739
PortEco:	atpI
STRING:	511145.b3739
COLOMBOS:	atpI

Product

Name:

ATP synthase accessory factor AtpI

Synonym(s):

AtpI, UncI

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

13.632

Isoelectric point:

12.164

Motif(s):

Type	Positions	Sequence
	38 -> 58	VSAISGGLAVFLPNVLFMIFA
	71 -> 94	VAWTFAFGEAFKVLAMLVLLVVAL
	2 -> 126	SVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGGLAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFLPLIVTWVLVLVVQILAPAVINNKG
	13 -> 108	KLLLVQLLVVIASGLLFSLKDPFWGVSAISGGLAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFLPLIVTW
	101 -> 117	FLPLIVTWVLVLVVQIL

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.8 - ATP proton motive force interconversion
		4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.2 - The H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) Su
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0016021 - integral component of membrane GO:0045263 - proton-transporting ATP synthase complex, coupling factor F(o)
	biological_process	GO:0006811 - ion transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Brusilow WS., et al., 1983
[2] Hartmann C., et al., 2011
[3] Kasimoglu E., et al., 1996
[4] Porter AC., et al., 1983
[5] Schneppe B., et al., 1991
[6] Schneppe B., et al., 1990
[7] von Meyenburg K., et al., 1982

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

atpIBEFHAGDC

Operon arrangement:

Transcription unit	Promoter
atpBEFHAGDC	atpBp2
atpBEFHAGDC	atpBp1
atpIBEFHAGDC	atpIp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_4330 (cluster)	3921379	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3921378 to 3921379, where the highest frequency is at position 3919401. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4331	3921382	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_4332 (cluster)	3921385	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3921384 to 3921385, where the highest frequency is at position 3919408. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4333	3921390	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_4334	3921401	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_4335	3921709	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_4336	3921775	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_4337 (cluster)	3921812	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3921811 to 3921812, where the highest frequency is at position 3919835. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4338 (cluster)	3921818	reverse	For this promoter, there is a cluster of 4 transcription start sites,in the interval 3921815 to 3921818, where the highest frequency is at position 3919840. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4339 (cluster)	3921823	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3921822 to 3921823, where the highest frequency is at position 3919846. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4340 (cluster)	3921829	reverse	For this promoter, there is a cluster of 3 transcription start sites,in the interval 3921827 to 3921829, where the highest frequency is at position 3919852. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4341 (cluster)	3922078	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3922077 to 3922078, where the highest frequency is at position 3920100. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4342 (cluster)	3922116	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3922115 to 3922116, where the highest frequency is at position 3920139. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[8]
promoter	TSS_4343	3922124	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_4344	3922273	reverse	nd	[RS-EPT-CBR]	[8]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Brusilow WS., Porter AC., Simoni RD., 1983, Cloning and expression of uncI, the first gene of the unc operon of Escherichia coli., J Bacteriol 155(3):1265-70
[2] Hartmann C., Engel A., 2011, Cloning, expression, purification, and characterization of the membrane protein UncI from Escherichia coli., Protein Expr Purif 79(2):187-90
[3] Kasimoglu E., Park SJ., Malek J., Tseng CP., Gunsalus RP., 1996, Transcriptional regulation of the proton-translocating ATPase (atpIBEFHAGDC) operon of Escherichia coli: control by cell growth rate., J Bacteriol 178(19):5563-7
[4] Porter AC., Brusilow WS., Simoni RD., 1983, Promoter for the unc operon of Escherichia coli., J Bacteriol 155(3):1271-8
[5] Schneppe B., Deckers-Hebestreit G., Altendorf K., 1991, Detection and localization of the i protein in Escherichia coli cells using antibodies., FEBS Lett 292(1-2):145-7
[6] Schneppe B., Deckers-Hebestreit G., Altendorf K., 1990, Overproduction and purification of the uncI gene product of the ATP synthase of Escherichia coli., J Biol Chem 265(1):389-95
[7] von Meyenburg K., Jorgensen BB., Nielsen J., Hansen FG., 1982, Promoters of the atp operon coding for the membrane-bound ATP synthase of Escherichia coli mapped by Tn10 insertion mutations., Mol Gen Genet 188(2):240-8
[8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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