RegulonDB

Gene
Name:	betI
Synonym(s):	ECK0311, EG10111, b0313
Genome position(nucleotides):	328747 <-- 329334
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.8
External database links:

ASAP:	ABE-0001076
CGSC:	30692
ECHOBASE:	EB0109
ECOLIHUB:	betI
OU-MICROARRAY:	b0313
STRING:	511145.b0313
COLOMBOS:	betI

Product

Name:

DNA-binding transcriptional repressor BetI

Synonym(s):

BetI

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

TetR/AcrR

Cellular location:

cytosol

Molecular weight:

21.815

Isoelectric point:

10.716

Motif(s):

Type	Positions	Sequence	Comment
	8 -> 68	SIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTGIISHYFRDKNGLLEATMRDITSQL	UniProt: HTH tetR-type.
	14 -> 59	LIDATLEAINEVGMHDATIAQIARRAGVSTGIISHYFRDKNGLLEA
	31 -> 50	TIAQIARRAGVSTGIISHYF	UniProt: H-T-H motif.
	85 -> 192	EQRLQAIVGGNFDETQVSSAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAALIDGLWLRAALSGKPLDKTRANSLTRHFITQHL

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
		5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0003700 - DNA-binding transcription factor activity GO:0000976 - transcription cis-regulatory region binding
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0006970 - response to osmotic stress GO:0045892 - negative regulation of transcription, DNA-templated GO:0019285 - glycine betaine biosynthetic process from choline

Note(s):

Note(s): ...[more].

Evidence:

[COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity
[EXP-IEP] Inferred from expression pattern

Reference(s):

[1] Lamark T., et al., 1991
[2] Rkenes TP., et al., 1996

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	Cra
Repressed by:	BetI, ArcA

Reference(s)
[1] Lamark T., Kaasen I., Eshoo MW., Falkenberg P., McDougall J., Strom AR., 1991, DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli., Mol Microbiol 5(5):1049-64
[2] Rkenes TP., Lamark T., Strom AR., 1996, DNA-binding properties of the BetI repressor protein of Escherichia coli: the inducer choline stimulates BetI-DNA complex formation., J Bacteriol 178(6):1663-70
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78