RegulonDB

Gene
Name:	betI
Synonym(s):	ECK0311, EG10111, b0313
Genome position(nucleotides):	328747 <-- 329334 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.8
External database links:

ASAP:	ABE-0001076
CGSC:	30692
ECHOBASE:	EB0109
ECOLIHUB:	betI
OU-MICROARRAY:	b0313
STRING:	511145.b0313
COLOMBOS:	betI

Product

Name:

DNA-binding transcriptional repressor BetI

Synonym(s):

BetI

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

TetR/AcrR

Cellular location:

cytosol

Molecular weight:

21.815

Isoelectric point:

10.716

Motif(s):

Type	Positions	Sequence
	8 -> 68	SIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTGIISHYFRDKNGLLEATMRDITSQL
	31 -> 50	TIAQIARRAGVSTGIISHYF
	85 -> 192	EQRLQAIVGGNFDETQVSSAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAALIDGLWLRAALSGKPLDKTRANSLTRHFITQHL
	14 -> 59	LIDATLEAINEVGMHDATIAQIARRAGVSTGIISHYFRDKNGLLEA

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
		5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0003700 - DNA-binding transcription factor activity GO:0000976 - transcription regulatory region sequence-specific DNA binding
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0006970 - response to osmotic stress GO:0045892 - negative regulation of transcription, DNA-templated GO:0019285 - glycine betaine biosynthetic process from choline

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[HIFS] Human inference of function from sequence
[IEP] Inferred from expression pattern

Reference(s):

[1] Lamark T., et al., 1991
[2] Rkenes TP., et al., 1996

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

betIBA

Operon arrangement:

Transcription unit	Promoter
betIBA	betIp

Transcriptional Regulation
	Display Regulation
Activated by:	Cra
Repressed by:	BetI, ArcA

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	betAp5	327261		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.455. P-value: 4.108e-5 Read more >	[ICWHO]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Lamark T., Kaasen I., Eshoo MW., Falkenberg P., McDougall J., Strom AR., 1991, DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli., Mol Microbiol 5(5):1049-64
[2] Rkenes TP., Lamark T., Strom AR., 1996, DNA-binding properties of the BetI repressor protein of Escherichia coli: the inducer choline stimulates BetI-DNA complex formation., J Bacteriol 178(6):1663-70
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78