RegulonDB RegulonDB 11.1: Gene Form
   

bioA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

bioA bioB ybhB bioF BirA terminator bioBp bioBp bioAp bioAp TSS_941 TSS_941 ybhBp2 ybhBp2 ybhBp4 ybhBp4

Gene      
Name: bioA    Texpresso search in the literature
Synonym(s): ECK0763, EG10117, b0774
Genome position(nucleotides): 807968 <-- 809257
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.28
External database links:  
ASAP:
 ABE-0002635
CGSC:
 959
ECHOBASE:
 EB0115
ECOLIHUB:
 bioA
OU-MICROARRAY:
 b0774
STRING:
 511145.b0774
COLOMBOS: bioA


Product      
Name: adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Synonym(s): BioA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 47.336
Isoelectric point: 5.703
Motif(s):
 
Type Positions Sequence Comment
11 -> 11 R UniProt: In Ref. 2; CAA00964..
17 -> 17 Y UniProt: Severely reduces the aminotransferase activity..
26 -> 420 VYPVVSAEGCELILSDGRRLVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQPLECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNSMHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAGGMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKALTGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQQVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRPFGKLIYLMPPYIILPQQLQRLTAAVNRA
99 -> 102 TPQP UniProt: In Ref. 1; AAA23514..
112 -> 113 GS UniProt: Pyridoxal phosphate binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.1 - biotin
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0004015 - adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0008483 - transaminase activity
GO:0030170 - pyridoxal phosphate binding
GO:0042803 - protein homodimerization activity
biological_process GO:0009102 - biotin biosynthetic process
External database links:  
ALPHAFOLD:
 P12995
DIP:
 DIP-9219N
ECOCYC:
 DAPASYN-MONOMER
ECOLIWIKI:
 b0774
INTERPRO:
 IPR015422
INTERPRO:
 IPR015424
INTERPRO:
 IPR015421
INTERPRO:
 IPR005815
INTERPRO:
 IPR005814
MODBASE:
 P12995
PDB:
 1QJ3
PDB:
 6ED7
PDB:
 1S09
PDB:
 1S0A
PDB:
 1QJ5
PDB:
 1S06
PDB:
 1S07
PDB:
 1S08
PDB:
 1DTY
PDB:
 1MGV
PDB:
 1MLZ
PDB:
 1MLY
PFAM:
 PF00202
PRIDE:
 P12995
PRODB:
 PRO_000022221
PROSITE:
 PS00600
REFSEQ:
 NP_415295
SMR:
 P12995
UNIPROT:
 P12995


Operon      
Name: bioA         
Operon arrangement:
Transcription unit        Promoter
bioA


Transcriptional Regulation      
Display Regulation             
Repressed by: BirA


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybhBp4 807930 reverse nd [COMP-AINF] [1]
  promoter ybhBp2 808035 reverse nd [COMP-AINF] [1]
  promoter TSS_941 809290 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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