RegulonDB RegulonDB 11.1: Gene Form
   

cysN gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cysN cysD cysC ygbE TSS_3040 TSS_3040 TSS_3039 TSS_3039 cysCp3 cysCp3 ygbEp3 ygbEp3

Gene      
Name: cysN    Texpresso search in the literature
Synonym(s): ECK2746, EG10194, b2751
Genome position(nucleotides): 2873992 <-- 2875419
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.62
External database links:  
ASAP:
 ABE-0009026
CGSC:
 18436
ECHOBASE:
 EB0191
ECOLIHUB:
 cysN
OU-MICROARRAY:
 b2751
STRING:
 511145.b2751
COLOMBOS: cysN


Product      
Name: sulfate adenylyltransferase subunit 1
Synonym(s): CysN, sulfate adenylyltransferase, CysN subunit
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 52.558
Isoelectric point: 4.749
Motif(s):
 
Type Positions Sequence Comment
25 -> 239 KSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEVLETVEIQR UniProt: tr-type G.
27 -> 231 LLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGPTLLEV
34 -> 41 GSVDDGKS UniProt: GTP.
92 -> 96 GITID UniProt: G2; Sequence Annotation Type: region of interest.
113 -> 117 DTPGH UniProt: GTP.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.2 - sulfur metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0009336 - sulfate adenylyltransferase complex (ATP)
GO:0005829 - cytosol
molecular_function GO:0016779 - nucleotidyltransferase activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0000166 - nucleotide binding
GO:0003924 - GTPase activity
GO:0005524 - ATP binding
GO:0005525 - GTP binding
GO:0004781 - sulfate adenylyltransferase (ATP) activity
biological_process GO:0006790 - sulfur compound metabolic process
GO:0000103 - sulfate assimilation
GO:0019419 - sulfate reduction
GO:0070814 - hydrogen sulfide biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P23845
DIP:
 DIP-513N
ECOCYC:
 CYSN-MONOMER
ECOLIWIKI:
 b2751
INTERPRO:
 IPR027417
INTERPRO:
 IPR031157
INTERPRO:
 IPR041757
INTERPRO:
 IPR044138
INTERPRO:
 IPR009000
INTERPRO:
 IPR044139
INTERPRO:
 IPR005225
INTERPRO:
 IPR000795
INTERPRO:
 IPR009001
INTERPRO:
 IPR011779
MODBASE:
 P23845
PFAM:
 PF00009
PRIDE:
 P23845
PRINTS:
 PR00315
PRODB:
 PRO_000022384
PROSITE:
 PS51722
PROSITE:
 PS00301
REFSEQ:
 NP_417231
SMR:
 P23845
SWISSMODEL:
 P23845
UNIPROT:
 P23845


Operon      
Name: cysDNC         
Operon arrangement:
Transcription unit        Promoter
cysDNC


Transcriptional Regulation      
Display Regulation             
Activated by: CysB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ygbEp3 2873392 reverse nd [COMP-AINF] [1]
  promoter cysCp3 2874028 reverse nd [COMP-AINF] [1]
  promoter TSS_3039 2875496 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3040 2875544 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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