RegulonDB RegulonDB 11.1: Gene Form
   

dnaB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dnaB alr qorA pspH ppGpp terminator anti-terminator alrp2 alrp2 alrp1 alrp1 qorAp qorAp TSS_4887 (cluster) TSS_4887 (cluster) dnaBp dnaBp

Gene      
Name: dnaB    Texpresso search in the literature
Synonym(s): ECK4044, EG10236, b4052, groP, grpA, grpD
Genome position(nucleotides): 4264314 --> 4265729
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.81
External database links:  
ASAP:
 ABE-0013269
CGSC:
 850
ECHOBASE:
 EB0232
ECOLIHUB:
 dnaB
OU-MICROARRAY:
 b4052
STRING:
 511145.b4052
COLOMBOS: dnaB


Product      
Name: replicative DNA helicase
Synonym(s): DnaB, GroP, GrpA, GrpD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 52.39
Isoelectric point: 4.673
Motif(s):
 
Type Positions Sequence Comment
1 -> 27 MAGNKPFNKQQAEPRERDPQVAGLKVP UniProt: Disordered; Sequence Annotation Type: region of interest.
27 -> 128 PPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRE
200 -> 467 PHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQ UniProt: SF4 helicase.
206 -> 462 GVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDN
231 -> 238 ARPSMGKT UniProt: ATP.

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related --> 2.1.1 - DNA replication
Gene Ontology Terms (GO)  
cellular_component GO:1990158 - DnaB-DnaC-DnaT-PriA-PriB complex
GO:1990159 - DnaB-DnaC-DnaT-PriA-PriC complex
GO:1990156 - DnaB-DnaG complex
GO:1990160 - DnaB-DnaC-Rep-PriC complex
GO:1990100 - DnaB-DnaC complex
GO:0005829 - cytosol
GO:0030894 - replisome
GO:0033202 - DNA helicase complex
GO:1990077 - primosome complex
molecular_function GO:0003677 - DNA binding
GO:0003678 - DNA helicase activity
GO:0004386 - helicase activity
GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0016887 - ATP hydrolysis activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0042802 - identical protein binding
biological_process GO:0006260 - DNA replication
GO:0006268 - DNA unwinding involved in DNA replication
GO:0006270 - DNA replication initiation
GO:0032508 - DNA duplex unwinding
GO:0006269 - DNA replication, synthesis of RNA primer
GO:0010212 - response to ionizing radiation
GO:0031297 - replication fork processing
External database links:  
ALPHAFOLD:
 P0ACB0
DIP:
 DIP-9456N
ECOCYC:
 EG10236-MONOMER
ECOLIWIKI:
 b4052
INTERPRO:
 IPR007692
INTERPRO:
 IPR007693
INTERPRO:
 IPR007694
INTERPRO:
 IPR016136
INTERPRO:
 IPR027417
INTERPRO:
 IPR003593
INTERPRO:
 IPR036185
MINT:
 P0ACB0
MODBASE:
 P0ACB0
PDB:
 6QEM
PDB:
 1JWE
PDB:
 6BBM
PDB:
 6KZA
PDB:
 6QEL
PDB:
 1B79
PFAM:
 PF03796
PFAM:
 PF00772
PRIDE:
 P0ACB0
PRODB:
 PRO_000022460
PROSITE:
 PS51199
REFSEQ:
 NP_418476
SMART:
 SM00382
SMR:
 P0ACB0
SWISSMODEL:
 P0ACB0
UNIPROT:
 P0ACB0


Operon      
Name: dnaB         
Operon arrangement:
Transcription unit        Promoter
dnaB


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4887 (cluster) 4264284 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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