RegulonDB RegulonDB 11.1: Gene Form
   

eda gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

edd purT eda GntR KdgR PhoB anti-anti-terminator anti-terminator edap1 edap1 edap2 edap2 edap3 edap3 TSS_2193 TSS_2193 TSS_2192 TSS_2192 TSS_2191 TSS_2191 TSS_2190 TSS_2190

Gene      
Name: eda    Texpresso search in the literature
Synonym(s): ECK1851, EG10256, b1850, hga, kdgA, kga
Genome position(nucleotides): 1932115 <-- 1932756
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.23
Reference(s): [1] Carter AT., et al., 1993
[2] Conway T., et al., 1991
[3] Egan SE., et al., 1992
External database links:  
ASAP:
 ABE-0006164
CGSC:
 826
ECHOBASE:
 EB0252
ECOLIHUB:
 eda
OU-MICROARRAY:
 b1850
STRING:
 511145.b1850
COLOMBOS: eda


Product      
Name: KHG/KDPG aldolase
Synonym(s): Eda, Hga, KdgA, Kga
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 22.284
Isoelectric point: 5.347
Motif(s):
 
Type Positions Sequence Comment
8 -> 9 AE UniProt: Substrate binding; Sequence Annotation Type: region of interest.
9 -> 203 ESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKL
45 -> 45 E UniProt: 50-fold decrease in catalytic efficiency and 6-fold decrease of binding affinity..
133 -> 133 K UniProt: Absence of aldolase activity. Shows a markedly altered substrate specificity relative to the wild-type, with an enhanced activity against pyridine carboxaldehyde, benzaldehyde, and alpha-ketobutyrate; when associated with k-161..
161 -> 161 T UniProt: Little stereoselectivity, accepting KDPG and KDPGal as substrate with roughly equal efficacy. It strongly diminishes the activity against KDPG and slightly increases activity against KDPGal..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.9 - Entner-Doudoroff
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.21 - glyoxylate degradation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
molecular_function GO:0106009 - (4S)-4-hydroxy-2-oxoglutarate aldolase activity
GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0016833 - oxo-acid-lyase activity
GO:0008675 - 2-dehydro-3-deoxy-phosphogluconate aldolase activity
GO:0008700 - 4-hydroxy-2-oxoglutarate aldolase activity
GO:0008948 - oxaloacetate decarboxylase activity
GO:0042802 - identical protein binding
biological_process GO:0008152 - metabolic process
GO:0009255 - Entner-Doudoroff pathway through 6-phosphogluconate
Reference(s): [4] Faik P., et al., 1971
[5] Fuhrman LK., et al., 1998
External database links:  
ALPHAFOLD:
 P0A955
DIP:
 DIP-36196N
ECOCYC:
 KDPGALDOL-4OH2OXOGLUTARALDOL-MONOMER
ECOLIWIKI:
 b1850
INTERPRO:
 IPR031337
INTERPRO:
 IPR031338
INTERPRO:
 IPR013785
INTERPRO:
 IPR000887
MODBASE:
 P0A955
PANTHER:
 PTHR30246
PDB:
 1WAU
PDB:
 1FWR
PDB:
 1WBH
PDB:
 2C0A
PDB:
 1EUA
PDB:
 1EUN
PDB:
 1FQ0
PFAM:
 PF01081
PRIDE:
 P0A955
PRODB:
 PRO_000022502
PROSITE:
 PS00159
PROSITE:
 PS00160
REFSEQ:
 NP_416364
SMR:
 P0A955
UNIPROT:
 P0A955


Operon      
Name: edd-eda         
Operon arrangement:
Transcription unit        Promoter
eda
eda
eda
edd-eda


Transcriptional Regulation      
Display Regulation             
Repressed by: KdgR, PhoB, Cra, GntR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2190 1931849 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2191 1932530 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2192 1932726 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2193 1932744 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Carter AT., Pearson BM., Dickinson JR., Lancashire WE., 1993, Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway., Gene 130(1):155-6

 [2] Conway T., Yi KC., Egan SE., Wolf RE., Rowley DL., 1991, Locations of the zwf, edd, and eda genes on the Escherichia coli physical map., J Bacteriol 173(17):5247-8

 [3] Egan SE., Fliege R., Tong S., Shibata A., Wolf RE., Conway T., 1992, Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon., J Bacteriol 174(14):4638-46

 [4] Faik P., Kornberg HL., McEvoy-Bowe E., 1971, Isolation and properties of Escherichia coli mutants defective in 2-keto 3-deoxy 6-phosphogluconate aldolase activity., FEBS Lett 19(3):225-228

 [5] Fuhrman LK., Wanken A., Nickerson KW., Conway T., 1998, Rapid accumulation of intracellular 2-keto-3-deoxy-6-phosphogluconate in an Entner-Doudoroff aldolase mutant results in bacteriostasis., FEMS Microbiol Lett 159(2):261-6

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB