RegulonDB

Gene
Name:	fabA
Synonym(s):	ECK0945, EG10273, b0954
Genome position(nucleotides):	1015952 <-- 1016470 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.18
Reference(s):	[1] Cronan JE., et al., 1988 [2] Cronan JE., et al., 1972 [3] Feng Y., et al., 2009 [4] Feng Y., et al., 2011 [5] Henry MF., et al., 1992 [6] Nunn WD., et al., 1983
External database links:

ASAP:	ABE-0003229
CGSC:	799
ECHOBASE:	EB0269
ECOLIHUB:	fabA
OU-MICROARRAY:	b0954
STRING:	511145.b0954
COLOMBOS:	fabA

Product

Name:

β-hydroxyacyl-acyl carrier protein dehydratase/isomerase

Synonym(s):

beta;-hydroxyacyl-ACP dehydratase/isomerase, FabA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

18.969

Isoelectric point:

6.532

Motif(s):

Type	Positions	Sequence
	2 -> 172	VDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMTETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEGKGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASDLKVGLFQDTSAF
	29 -> 158	LPAPNMLMMDRVVKMTETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEGKGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTA
	102 -> 102	G
	76 -> 76	P

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.4 - fatty acids and phosphatidic acid
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0008659 - (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity GO:0004317 - 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity GO:0016829 - lyase activity GO:0016853 - isomerase activity GO:0008693 - 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity GO:0019171 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity GO:0042803 - protein homodimerization activity GO:0034017 - trans-2-decenoyl-acyl-carrier-protein isomerase activity GO:0047451 - 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
	biological_process	GO:0006629 - lipid metabolic process GO:0006631 - fatty acid metabolic process GO:0008610 - lipid biosynthetic process GO:0006633 - fatty acid biosynthetic process

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PANTHER:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

fabA

Operon arrangement:

Transcription unit	Promoter
fabA	fabAp

Transcriptional Regulation
	Display Regulation
Activated by:	FadR
Repressed by:	FabR

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1249	1016472	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1250	1016488	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1251 (cluster)	1016497	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 1016496 to 1016497, where the highest frequency is at position 1015720. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[7]
promoter	TSS_1252	1016859	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1253	1017045	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1254	1017052	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1255 (cluster)	1017834	reverse	For this promoter, there is a cluster of 3 transcription start sites,in the interval 1017832 to 1017834, where the highest frequency is at position 1017057. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[7]
promoter	TSS_1256	1017837	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1257	1017843	reverse	nd	[RS-EPT-CBR]	[7]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Cronan JE., Li WB., Coleman R., Narasimhan M., de Mendoza D., Schwab JM., 1988, Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase., J Biol Chem 263(10):4641-6
[2] Cronan JE., Silbert DF., Wulff DL., 1972, Mapping of the fabA locus for unsaturated fatty acid biosynthesis in Escherichia coli., J Bacteriol 112(1):206-11
[3] Feng Y., Cronan JE., 2009, Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB., J Biol Chem 284(43):29526-35
[4] Feng Y., Cronan JE., 2011, Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters., Mol Microbiol 80(1):195-218
[5] Henry MF., Cronan JE., 1992, A new mechanism of transcriptional regulation: release of an activator triggered by small molecule binding., Cell 70(4):671-9
[6] Nunn WD., Giffin K., Clark D., Cronan JE., 1983, Role for fadR in unsaturated fatty acid biosynthesis in Escherichia coli., J Bacteriol 154(2):554-60
[7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.