RegulonDB RegulonDB 11.1: Gene Form
   

fepA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fepA fes entD CRP H-NS Fur Fur Fur Fur Fur FNR OmrB OmrA terminator fesp fesp fepAp fepAp entDp1 entDp1 entDp2 entDp2

Gene      
Name: fepA    Texpresso search in the literature
Synonym(s): ECK0576, EG10293, b0584, cbr, cbt, fep, feuB
Genome position(nucleotides): 610254 <-- 612494
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.08
External database links:  
ASAP:
 ABE-0002014
CGSC:
 18388
ECHOBASE:
 EB0289
ECOLIHUB:
 fepA
OU-MICROARRAY:
 b0584
STRING:
 511145.b0584
COLOMBOS: fepA


Product      
Name: ferric enterobactin outer membrane transporter
Synonym(s): Cbr, Cbt, Fep, FepA, FeuB
Sequence: Get amino acid sequence Fasta Format
Cellular location: outer membrane,cell envelope (sensu Bacteria)
Molecular weight: 82.107
Isoelectric point: 5.245
Motif(s):
 
Type Positions Sequence Comment
1 -> 22 MNKKIHSLALLVNLGIYGVAQA
23 -> 175 QEPTDTPVSHDDTIVVTAAEQNLQAPGVSTITADEIRKNPVARDVSKIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAAGGVVNIITKKGSGEW
34 -> 41 DTIVVTAA UniProt: TonB box; Sequence Annotation Type: short sequence motif.
36 -> 36 I Alternate sequence I → P; no detectable TonB-FepA complex in mutant strain
49 -> 163 GVSTITADEIRKNPVARDVSKIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAAGGV

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.1 - Channel-type Transporters --> 4.1.B - Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily)
  5 - cell processes --> 5.5 - adaptations --> 5.5.7 - Fe aquisition
  8 - extrachromosomal --> 8.4 - colicin related
Gene Ontology Terms (GO)  
cellular_component GO:0009279 - cell outer membrane
GO:0016020 - membrane
GO:0030313 - cell envelope
GO:0016021 - integral component of membrane
GO:0031230 - intrinsic component of cell outer membrane
GO:0045203 - integral component of cell outer membrane
GO:1902495 - transmembrane transporter complex
molecular_function GO:0019904 - protein domain specific binding
GO:0005515 - protein binding
GO:0042912 - colicin transmembrane transporter activity
GO:0038023 - signaling receptor activity
GO:0022834 - ligand-gated channel activity
GO:0015620 - ferric-enterobactin transmembrane transporter activity
GO:0042931 - enterobactin transmembrane transporter activity
GO:0015344 - siderophore uptake transmembrane transporter activity
biological_process GO:0006811 - ion transport
GO:0055072 - iron ion homeostasis
GO:0015891 - siderophore transport
GO:0042930 - enterobactin transport
GO:0033214 - siderophore-dependent iron import into cell
GO:0042914 - colicin transport
GO:0015685 - ferric-enterobactin import into cell
GO:0044718 - siderophore transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Annamalai R., et al., 2004
[2] Cao Z., et al., 2000
[3] Chakraborty R., et al., 2003
[4] Cohen-Khait R., et al., 2021
[5] Ecker DJ., et al., 1986
[6] Guterman SK. 1971
[7] Lill Y., et al., 2016
[8] Majumdar A., et al., 2020
[9] Murphy CK., et al., 1990
[10] Pugsley AP., et al., 1977
[11] Pugsley AP., et al., 1976
[12] Smallwood CR., et al., 2009
[13] Thulasiraman P., et al., 1998
External database links:  
ALPHAFOLD:
 P05825
DIP:
 DIP-9592N
ECOCYC:
 EG10293-MONOMER
ECOLIWIKI:
 b0584
INTERPRO:
 IPR012910
INTERPRO:
 IPR036942
INTERPRO:
 IPR037066
INTERPRO:
 IPR010917
INTERPRO:
 IPR000531
INTERPRO:
 IPR010105
INTERPRO:
 IPR039426
INTERPRO:
 IPR010916
MODBASE:
 P05825
PANTHER:
 PTHR30069
PDB:
 1FEP
PFAM:
 PF07715
PFAM:
 PF00593
PRIDE:
 P05825
PRODB:
 PRO_000022600
PROSITE:
 PS01156
PROSITE:
 PS00430
REFSEQ:
 NP_415116
SMR:
 P05825
UNIPROT:
 P05825


Operon      
Name: fepA-entD         
Operon arrangement:
Transcription unit        Promoter
fepA-entD


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: Fur


RNA cis-regulatory element    
Attenuation: Translational


Regulation by small RNA    
  Display Regulation
small RNA omrA, omrB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter entDp2 610141 reverse nd [COMP-AINF] [14]
  promoter entDp1 610254 reverse nd [COMP-AINF] [14]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Annamalai R., Jin B., Cao Z., Newton SM., Klebba PE., 2004, Recognition of ferric catecholates by FepA., J Bacteriol 186(11):3578-89

 [2] Cao Z., Qi Z., Sprencel C., Newton SM., Klebba PE., 2000, Aromatic components of two ferric enterobactin binding sites in Escherichia coli FepA., Mol Microbiol 37(6):1306-17

 [3] Chakraborty R., Lemke EA., Cao Z., Klebba PE., van der Helm D., 2003, Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli., Biometals 16(4):507-18

 [4] Cohen-Khait R., Harmalkar A., Pham P., Webby MN., Housden NG., Elliston E., Hopper JTS., Mohammed S., Robinson CV., Gray JJ., Kleanthous C., 2021, Colicin-Mediated Transport of DNA through the Iron Transporter FepA., mBio :e0178721

 [5] Ecker DJ., Matzanke BF., Raymond KN., 1986, Recognition and transport of ferric enterobactin in Escherichia coli., J Bacteriol 167(2):666-73

 [6] Guterman SK., 1971, Inhibition of colicin B by enterochelin., Biochem Biophys Res Commun 44(5):1149-55

 [7] Lill Y., Jordan LD., Smallwood CR., Newton SM., Lill MA., Klebba PE., Ritchie K., 2016, Confined Mobility of TonB and FepA in Escherichia coli Membranes., PLoS One 11(12):e0160862

 [8] Majumdar A., Trinh V., Moore KJ., Smallwood CR., Kumar A., Yang T., Scott DC., Long NJ., Newton SM., Klebba PE., 2020, Conformational rearrangements in the N-domain of Escherichia coli FepA during ferric enterobactin transport., J Biol Chem 295(15):4974-4984

 [9] Murphy CK., Kalve VI., Klebba PE., 1990, Surface topology of the Escherichia coli K-12 ferric enterobactin receptor., J Bacteriol 172(5):2736-46

 [10] Pugsley AP., Reeves P., 1977, Uptake of ferrienterochelin by Escherichia coli: energy dependent stage of uptake., J Bacteriol 130(1):26-36

 [11] Pugsley AP., Reeves P., 1976, Iron uptake in colicin B-resistant mutants of Escherichia coli K-12., J Bacteriol 126(3):1052-62

 [12] Smallwood CR., Marco AG., Xiao Q., Trinh V., Newton SM., Klebba PE., 2009, Fluoresceination of FepA during colicin B killing: effects of temperature, toxin and TonB., Mol Microbiol 72(5):1171-80

 [13] Thulasiraman P., Newton SM., Xu J., Raymond KN., Mai C., Hall A., Montague MA., Klebba PE., 1998, Selectivity of ferric enterobactin binding and cooperativity of transport in gram-negative bacteria., J Bacteriol 180(24):6689-96

 [14] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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