RegulonDB RegulonDB 11.1: Gene Form
   

fhlA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fhlA hypE ygbA hypD OxyS OxyS terminator

Gene      
Name: fhlA    Texpresso search in the literature
Synonym(s): ECK2726, EG10301, b2731, fhl
Genome position(nucleotides): 2854338 --> 2856416
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.57
External database links:  
ASAP:
 ABE-0008971
CGSC:
 18370
ECHOBASE:
 EB0297
ECOLIHUB:
 fhlA
OU-MICROARRAY:
 b2731
STRING:
 511145.b2731
COLOMBOS: fhlA


Shine dalgarno      
Sequence: tgctttcctgGAAGAAcaaaATG


Product      
Name: DNA-binding transcriptional activator FhlA
Synonym(s): Fhl, FhlA, formate hydrogen-lyase transcriptional activator for fdhF, hyc and hyp operons
Sequence: Get amino acid sequence Fasta Format
Regulator Family: EBP
Cellular location: cytosol
Molecular weight: 78.468
Isoelectric point: 5.321
Motif(s):
 
Type Positions Sequence Comment
39 -> 49 KRSALADNAAI UniProt: In Ref. 2; AAA23770..
59 -> 71 ASYYASREKDTPI UniProt: In Ref. 2; AAA23770..
202 -> 344 DMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLRQIAERVAIAV UniProt: GAF.
210 -> 210 V UniProt: In Ref. 2; AAA23770..
284 -> 333 PYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLL UniProt: In Ref. 2; AAA23770..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
  3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)  
cellular_component GO:0005667 - transcription regulator complex
GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0005515 - protein binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0043565 - sequence-specific DNA binding
GO:0042802 - identical protein binding
GO:0000987 - cis-regulatory region sequence-specific DNA binding
biological_process GO:0006351 - transcription, DNA-templated
GO:0006355 - regulation of transcription, DNA-templated
GO:0000160 - phosphorelay signal transduction system
GO:2000144 - positive regulation of DNA-templated transcription, initiation
Reference(s): [1] Maupin JA., et al., 1990
[2] Schlensog V., et al., 1990
[3] Schlensog V., et al., 1994
External database links:  
ALPHAFOLD:
 P19323
ECOCYC:
 PD02936
ECOLIWIKI:
 b2731
INTERPRO:
 IPR025662
INTERPRO:
 IPR002078
INTERPRO:
 IPR003593
INTERPRO:
 IPR003018
INTERPRO:
 IPR002197
INTERPRO:
 IPR025944
INTERPRO:
 IPR027417
INTERPRO:
 IPR029016
INTERPRO:
 IPR009057
MODBASE:
 P19323
PFAM:
 PF00158
PFAM:
 PF01590
PRIDE:
 P19323
PRINTS:
 PR01590
PRODB:
 PRO_000022608
PROSITE:
 PS00675
PROSITE:
 PS50045
PROSITE:
 PS00688
REFSEQ:
 NP_417211
SMART:
 SM00382
SMART:
 SM00065
SMR:
 P19323
SWISSMODEL:
 P19323
UNIPROT:
 P19323


Operon      
Name: hypABCDE-fhlA         
Operon arrangement:
Transcription unit        Promoter
hypABCDE
hypABCDE-fhlA
hypBCDE
hypBCDE-fhlA


Transcriptional Regulation      
Display Regulation             
Activated by: FhlA, FNR, IHF
Repressed by: NsrR


RNA cis-regulatory element    
Attenuation: Translational


Regulation by small RNA    
  Display Regulation
small RNA oxyS, oxyS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Maupin JA., Shanmugam KT., 1990, Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulatory gene, fhlB., J Bacteriol 172(9):4798-806

 [2] Schlensog V., Bock A., 1990, Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli., Mol Microbiol 4(8):1319-27

 [3] Schlensog V., Lutz S., Bock A., 1994, Purification and DNA-binding properties of FHLA, the transcriptional activator of the formate hydrogenlyase system from Escherichia coli., J Biol Chem 269(30):19590-6

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