RegulonDB RegulonDB 11.1: Gene Form
   

fimE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fimB fimE fimA Lrp Lrp Lrp IHF IHF RseX terminator anti-terminator anti-anti-terminator TSS_5136 TSS_5136 TSS_5135 TSS_5135 TSS_5134 TSS_5134 TSS_5133 (cluster) TSS_5133 (cluster) TSS_5132 TSS_5132 fimAp fimAp fimEp fimEp

Gene      
Name: fimE    Texpresso search in the literature
Synonym(s): ECK4304, EG10312, b4313, hyp, pilH
Genome position(nucleotides): 4542037 --> 4542633
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 46.9
External database links:  
ASAP:
 ABE-0014143
CGSC:
 18346
ECHOBASE:
 EB0308
ECOLIHUB:
 fimE
OU-MICROARRAY:
 b4313
STRING:
 511145.b4313
COLOMBOS: fimE


Product      
Name: regulator for fimA
Synonym(s): FimE, Hyp, PilH
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 23.116
Isoelectric point: 10.16
Motif(s):
 
Type Positions Sequence Comment
2 -> 184 SKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISELLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAIFISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYLGHRNIRHTVRYTASNAARFAGLWE UniProt: Tyr recombinase.
6 -> 176 YLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISELLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAIFISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYLGHRNIRHTVRYTASNA

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.13 - fimbri, pili
  2 - information transfer --> 2.1 - DNA related --> 2.1.3 - DNA recombination
  3 - regulation --> 3.1 - type of regulation --> 3.1.1 - DNA structure level
  3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
  6 - cell structure --> 6.5 - pilus
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
biological_process GO:0006310 - DNA recombination
GO:0015074 - DNA integration
Note(s): Note(s): ...[more].
Evidence: [EXP-IDA-UNPURIFIED-PROTEIN] Assay of unpurified protein
Reference(s): [1] Breines DM., et al., 1994
[2] Gally DL., et al., 1996
[3] Hasman H., et al., 2000
[4] Teng CH., et al., 2006
[5] Wolf DM., et al., 2002
External database links:  
ALPHAFOLD:
 P0ADH7
ECOCYC:
 EG10312-MONOMER
ECOLIWIKI:
 b4313
INTERPRO:
 IPR002104
INTERPRO:
 IPR011010
INTERPRO:
 IPR013762
MODBASE:
 P0ADH7
PFAM:
 PF00589
PRIDE:
 P0ADH7
PRODB:
 PRO_000022620
PROSITE:
 PS51898
REFSEQ:
 NP_418733
SMR:
 P0ADH7
UNIPROT:
 P0ADH7


Operon      
Name: fimE         
Operon arrangement:
Transcription unit        Promoter
fimE


Transcriptional Regulation      
Display Regulation             
Activated by: LrhA, Lrp
Repressed by: H-NS


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_5132 4542983 forward nd [RS-EPT-CBR] [6]
  promoter TSS_5133 (cluster) 4543095 forward nd [RS-EPT-CBR] [6]
  promoter TSS_5134 4543103 forward nd [RS-EPT-CBR] [6]
  promoter TSS_5135 4543121 forward nd [RS-EPT-CBR] [6]
  promoter TSS_5136 4543128 forward nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Breines DM., Burnham JC., 1994, Modulation of Escherichia coli type 1 fimbrial expression and adherence to uroepithelial cells following exposure of logarithmic phase cells to quinolones at subinhibitory concentrations., J Antimicrob Chemother 34(2):205-21

 [2] Gally DL., Leathart J., Blomfield IC., 1996, Interaction of FimB and FimE with the fim switch that controls the phase variation of type 1 fimbriae in Escherichia coli K-12., Mol Microbiol 21(4):725-38

 [3] Hasman H., Schembri MA., Klemm P., 2000, Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12., J Bacteriol 182(4):1089-95

 [4] Teng CH., Xie Y., Shin S., Di Cello F., Paul-Satyaseela M., Cai M., Kim KS., 2006, Effects of ompA deletion on expression of type 1 fimbriae in Escherichia coli K1 strain RS218 and on the association of E. coli with human brain microvascular endothelial cells., Infect Immun 74(10):5609-16

 [5] Wolf DM., Arkin AP., 2002, Fifteen minutes of fim: control of type 1 pili expression in E. coli., OMICS 6(1):91-114

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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