RegulonDB

RegulonDB RegulonDB 10.9: Gene Form

ftsW gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	ftsW
Synonym(s):	ECK0090, EG10344, b0089
Genome position(nucleotides):	98403 --> 99647 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.01
External database links:

ASAP:	ABE-0000320
CGSC:	30409
ECHOBASE:	EB0340
ECOLIHUB:	ftsW
OU-MICROARRAY:	b0089
STRING:	511145.b0089
COLOMBOS:	ftsW

Product

Name:

essential cell division protein FtsW

Synonym(s):

FtsW, putative lipid II flippase FtsW

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

45.987

Isoelectric point:

10.218

Motif(s):

Type	Positions	Sequence
	48 -> 68	LLWLTFGLAAIGFIMVTSASM
	112 -> 132	ATMLLGSIILLMIVLVVGSSV
	175 -> 194	LRGFLKPMGVILVLAVLLLA
	311 -> 311	G
	402 -> 402	R

Type	Positions	Sequence
	48 -> 68	LLWLTFGLAAIGFIMVTSASM
	112 -> 132	ATMLLGSIILLMIVLVVGSSV
	175 -> 194	LRGFLKPMGVILVLAVLLLA
	311 -> 311	G
	402 -> 402	R
	150 -> 152	ELT
	172 -> 172	R
	343 -> 363	FLACSIGIWFSFQALVNVGAA
	368 -> 368	P
	181 -> 181	P
	198 -> 217	LGTVVVLFVTTLAMLFLAGA
	222 -> 244	FIAIIGMGISAVVLLILAEPYRI
	375 -> 375	P
	13 -> 33	LPGFSILVWISTALKGWVMGS
	243 -> 243	R
	302 -> 322	IIGEELGYVGVVLALLMVFFV
	87 -> 107	VYLILAFILAIITLRLPMEFW
	154 -> 156	LSL
	47 -> 402	TLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRLPMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYIANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAKLWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGELWGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKALEIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIMMLLRIDYETR
	153 -> 153	K
	253 -> 253	P
	374 -> 394	LPLISYGGSSLLIMSTAIMML
	145 -> 145	R
	170 -> 170	E

Classification:

Multifun Terms (GenProtEC)
		5 - cell processes --> 5.1 - cell division
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane GO:0032153 - cell division site
	molecular_function	GO:0005515 - protein binding GO:0016757 - transferase activity, transferring glycosyl groups GO:0016740 - transferase activity GO:0008955 - peptidoglycan glycosyltransferase activity GO:0015648 - lipid-linked peptidoglycan transporter activity
	biological_process	GO:0051301 - cell division GO:0007049 - cell cycle GO:0009252 - peptidoglycan biosynthetic process GO:0043093 - FtsZ-dependent cytokinesis GO:0008360 - regulation of cell shape GO:0071555 - cell wall organization GO:0015836 - lipid-linked peptidoglycan transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Ikeda M., et al., 1989
[2] Liu X., et al., 2018
[3] Ovchinnikov S., et al., 2015

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

PANTHER:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC

Operon arrangement:

Transcription unit	Promoter
mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC	mraZp
mraW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC	mraWp1
ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC	ftsLp1
ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC	ftsLp2
ftsQ	ftsQp2
ftsQAZ	ftsQp
ftsQAZ	ftsQp1
ftsAZ	ftsAp1
ftsZ	ftsZp4
ftsZ	ftsZp3
ftsZ	ftsZp2
lpxC	lpxCp1
lpxC	lpxCp2

Transcriptional Regulation
	Display Regulation
Repressed by:	MraZ, LexA, PdhR

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_162	96153	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_163 (cluster)	96971	forward	For this promoter, there is a cluster of 3 transcription start sites,in the interval 96971 to 96973, where the highest frequency is at position 96972. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[4]
promoter	TSS_164	96982	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_165	96984	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_166	96988	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_167	96993	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_168	96997	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_169	97532	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_170	97539	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_171	97550	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_172	100621	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_173 (cluster)	102049	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 102049 to 102050, where the highest frequency is at position 102049. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Ikeda M., Sato T., Wachi M., Jung HK., Ishino F., Kobayashi Y., Matsuhashi M., 1989, Structural similarity among Escherichia coli FtsW and RodA proteins and Bacillus subtilis SpoVE protein, which function in cell division, cell elongation, and spore formation, respectively., J Bacteriol 171(11):6375-8
[2] Liu X., Meiresonne NY., Bouhss A., den Blaauwen T., 2018, FtsW activity and lipid II synthesis are required for recruitment of MurJ to midcell during cell division in Escherichia coli., Mol Microbiol 109(6):855-884
[3] Ovchinnikov S., Kinch L., Park H., Liao Y., Pei J., Kim DE., Kamisetty H., Grishin NV., Baker D., 2015, Large-scale determination of previously unsolved protein structures using evolutionary information., Elife 4:e09248
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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