RegulonDB RegulonDB 11.1: Gene Form
   

fumA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fumA fumC manA tus CRP CRP CRP CRP CRP CRP CRP MarA SoxS Rob terminator terminator manAp manAp TSS_1895 TSS_1895 fumAp fumAp fumCp2 fumCp2 fumCp1 fumCp1

Gene      
Name: fumA    Texpresso search in the literature
Synonym(s): ECK1607, EG10356, b1612
Genome position(nucleotides): 1686731 <-- 1688377
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.55
External database links:  
ASAP:
 ABE-0005392
CGSC:
 18292
ECHOBASE:
 EB0351
ECOLIHUB:
 fumA
OU-MICROARRAY:
 b1612
STRING:
 511145.b1612
COLOMBOS: fumA


Product      
Name: fumarase A
Synonym(s): FumA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 60.299
Isoelectric point: 6.517
Motif(s):
 
Type Positions Sequence Comment
2 -> 548 SNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK UniProt: Fumarate hydratase class I, aerobic.
51 -> 326 ARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKA
332 -> 537 GIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQ

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0016853 - isomerase activity
GO:0046872 - metal ion binding
GO:0016836 - hydro-lyase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0004333 - fumarate hydratase activity
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
GO:0050163 - oxaloacetate tautomerase activity
biological_process GO:0006091 - generation of precursor metabolites and energy
GO:0006099 - tricarboxylic acid cycle
Note(s): Note(s): ...[more].
Reference(s): [1] Calderon IL., et al., 2009
[2] Gray CT., et al., 1966
External database links:  
ALPHAFOLD:
 P0AC33
DIP:
 DIP-36200N
ECOCYC:
 FUMA-MONOMER
ECOLIWIKI:
 b1612
INTERPRO:
 IPR004647
INTERPRO:
 IPR004646
INTERPRO:
 IPR020557
INTERPRO:
 IPR036660
INTERPRO:
 IPR011167
PFAM:
 PF05681
PFAM:
 PF05683
PRIDE:
 P0AC33
PRODB:
 PRO_000022734
PROSITE:
 PS00163
REFSEQ:
 NP_416129
SMR:
 P0AC33
UNIPROT:
 P0AC33


Operon      
Name: fumAC         
Operon arrangement:
Transcription unit        Promoter
fumC
fumC
fumA
fumAC


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: FNR, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1895 1688441 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Calderon IL., Elias AO., Fuentes EL., Pradenas GA., Castro ME., Arenas FA., Perez JM., Vasquez CC., 2009, Tellurite-mediated disabling of [4Fe-4S] clusters of Escherichia coli dehydratases., Microbiology 155(Pt 6):1840-6

 [2] Gray CT., Wimpenny JW., Mossman MR., 1966, Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli., Biochim Biophys Acta 117(1):33-41

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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