RegulonDB RegulonDB 10.9: Gene Form
   

ispG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hisS ispG rodZ sroE TSS_2816 TSS_2816 TSS_2815 TSS_2815 TSS_2814 (cluster) TSS_2814 (cluster) TSS_2813 (cluster) TSS_2813 (cluster) ispGp5 ispGp5 ispGp4 ispGp4 TSS_2812 TSS_2812 TSS_2811 (cluster) TSS_2811 (cluster) TSS_2810 TSS_2810 ispGp1 ispGp1 TSS_2809 (cluster) TSS_2809 (cluster) hisSp hisSp TSS_2808 TSS_2808 TSS_2807 TSS_2807 TSS_2806 TSS_2806 TSS_2805 TSS_2805 TSS_2804 (cluster) TSS_2804 (cluster) TSS_2803 TSS_2803 TSS_2802 TSS_2802 TSS_2801 TSS_2801 TSS_2800 TSS_2800 yfgMp1 yfgMp1 yfgMp2 yfgMp2 TSS_2799 (cluster) TSS_2799 (cluster) yfgMp3 yfgMp3 TSS_2798 TSS_2798

Gene      
Name: ispG    Texpresso search in the literature
Synonym(s): ECK2511, EG10370, b2515, gcpE
Genome position(nucleotides): 2640686 <-- 2641804 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.26
External database links:  
ASAP:
ABE-0008281
CGSC:
32909
ECHOBASE:
EB0365
ECOLIHUB:
ispG
OU-MICROARRAY:
b2515
STRING:
511145.b2515
COLOMBOS: ispG


Product      
Name: (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
Synonym(s): GcpE, IspG, flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 40.684
Isoelectric point: 6.084
Motif(s):
 
Type Positions Sequence
328 -> 328 G
335 -> 335 R
14 -> 353 RIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIR
322 -> 322 G

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.11 - menaquinone, ubiquinone
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0005506 - iron ion binding
biological_process GO:0044237 - cellular metabolic process
GO:0008299 - isoprenoid biosynthetic process
GO:0016114 - terpenoid biosynthetic process
GO:0019288 - isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0055114 - oxidation-reduction process
Note(s): Note(s): ...[more].
Reference(s): [1] Amslinger S., et al., 2002
[2] Liu YL., et al., 2012
[3] McAteer S., et al., 2001
[4] Nyland RL., et al., 2009
[5] Zhou K., et al., 2012
External database links:  
DIP:
DIP-35832N
ECOCYC:
EG10370-MONOMER
ECOLIWIKI:
b2515
INTERPRO:
IPR011005
INTERPRO:
IPR016425
INTERPRO:
IPR004588
MODBASE:
P62620
PANTHER:
PTHR30454
PFAM:
PF04551
PRIDE:
P62620
PRODB:
PRO_000023039
REFSEQ:
NP_417010
SMR:
P62620
UNIPROT:
P62620


Operon      
Name: ispG-sroE         
Operon arrangement:
Transcription unit        Promoter
ispG-sroE


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2798 2639305 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2799 (cluster) 2639374 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_2800 2639941 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2801 2639974 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2802 2640203 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2803 2640283 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2804 (cluster) 2640538 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_2805 2640541 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2806 2640554 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2807 2640563 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2808 2640565 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2809 (cluster) 2641405 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter ispGp1 2641880 reverse Similarity to the consensus
Read more >
[ICWHO] [7]
  promoter TSS_2810 2641922 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2811 (cluster) 2641929 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_2812 2641933 reverse nd [RS-EPT-CBR] [6]
  promoter ispGp4 2641950 reverse Similarity to the consensus
Read more >
[ICWHO] [7]
  promoter ispGp5 2641954 reverse Similarity to the consensus
Read more >
[ICWHO] [7]
  promoter TSS_2813 (cluster) 2641985 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_2814 (cluster) 2641990 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [6]
  promoter TSS_2815 2641997 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_2816 2642045 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Amslinger S., Kis K., Hecht S., Adam P., Rohdich F., Arigoni D., Bacher A., Eisenreich W., 2002, Biosynthesis of terpenes. Preparation of (E)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate, an intermediate of the deoxyxylulose phosphate pathway., J Org Chem 67(13):4590-4

 [2] Liu YL., Guerra F., Wang K., Wang W., Li J., Huang C., Zhu W., Houlihan K., Li Z., Zhang Y., Nair SK., Oldfield E., 2012, Structure, function and inhibition of the two- and three-domain 4Fe-4S IspG proteins., Proc Natl Acad Sci U S A 109(22):8558-63

 [3] McAteer S., Coulson A., McLennan N., Masters M., 2001, The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis., J Bacteriol 183(24):7403-7

 [4] Nyland RL., Xiao Y., Liu P., Freel Meyers CL., 2009, IspG converts an epoxide substrate analogue to (E)-4-hydroxy-3-methylbut-2-enyl diphosphate: implications for IspG catalysis in isoprenoid biosynthesis., J Am Chem Soc 131(49):17734-5

 [5] Zhou K., Zou R., Stephanopoulos G., Too HP., 2012, Metabolite profiling identified methylerythritol cyclodiphosphate efflux as a limiting step in microbial isoprenoid production., PLoS One 7(11):e47513

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [7] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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