RegulonDB RegulonDB 10.9: Gene Form
   

glyS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

glyS glyQ insJK insK sokA insJ TSS_4168 TSS_4168 TSS_4167 TSS_4167 glySp4 glySp4 TSS_4166 (cluster) TSS_4166 (cluster) TSS_4165 TSS_4165 TSS_4164 (cluster) TSS_4164 (cluster) TSS_4163 TSS_4163 TSS_4162 TSS_4162

Gene      
Name: glyS    Texpresso search in the literature
Synonym(s): ECK3547, EG10410, b3559, gly, gly-act, glyS(B)
Genome position(nucleotides): 3722328 <-- 3724397 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.41
External database links:  
ASAP:
ABE-0011624
CGSC:
674
ECHOBASE:
EB0405
ECOLIHUB:
glyS
OU-MICROARRAY:
b3559
STRING:
511145.b3559
COLOMBOS: glyS


Product      
Name: glycine—tRNA ligase subunit β
Synonym(s): Gly, GlyS, GlyS(B)
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 76.813
Isoelectric point: 5.08
Motif(s):
 
Type Positions Sequence
211 -> 212 ER
2 -> 2 S
91 -> 91 A
154 -> 156 DVH
342 -> 342 L

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.1 - amino acid -activation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016874 - ligase activity
GO:0004812 - aminoacyl-tRNA ligase activity
GO:0004820 - glycine-tRNA ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004814 - arginine-tRNA ligase activity
biological_process GO:0006412 - translation
GO:0006426 - glycyl-tRNA aminoacylation
GO:0006420 - arginyl-tRNA aminoacylation
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-6880N
ECOCYC:
GLYS-MONOMER
ECOLIWIKI:
b3559
INTERPRO:
IPR008909
INTERPRO:
IPR015944
INTERPRO:
IPR006194
PANTHER:
PTHR30075
PFAM:
PF05746
PFAM:
PF02092
PRIDE:
P00961
PRINTS:
PR01045
PRODB:
PRO_000022818
PROSITE:
PS50861
REFSEQ:
NP_418016
SMR:
P00961
UNIPROT:
P00961


Operon      
Name: glyQS         
Operon arrangement:
Transcription unit        Promoter
glyQS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4162 3721864 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4163 3722550 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4164 (cluster) 3723367 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4165 3723961 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4166 (cluster) 3724383 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter glySp4 3724495 reverse Similarity to the consensus
Read more >
[ICWHO] [2]
  promoter TSS_4167 3724689 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4168 3725309 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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