RegulonDB RegulonDB 11.1: Gene Form
   

ilvD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ilvD ilvA ilvE ilvAp ilvAp TSS_4395 TSS_4395 TSS_4394 TSS_4394 TSS_4393 TSS_4393

Gene      
Name: ilvD    Texpresso search in the literature
Synonym(s): ECK3763, EG10496, b3771
Genome position(nucleotides): 3953478 --> 3955328
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.35
External database links:  
ASAP:
 ABE-0012318
CGSC:
 606
ECHOBASE:
 EB0491
ECOLIHUB:
 ilvD
OU-MICROARRAY:
 b3771
STRING:
 511145.b3771
COLOMBOS: ilvD


Product      
Name: dihydroxy-acid dehydratase
Synonym(s): IlvD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 65.532
Isoelectric point: 5.677
Motif(s):
 
Type Positions Sequence Comment
2 -> 24 PKYRSATTTHGRNMAGARALWRA UniProt: In Ref. 2; AAA24023..
34 -> 607 KPIIAVVNSFTQFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIGILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFSQDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTGPAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKACALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELAARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGA
164 -> 164 L UniProt: In Ref. 2; AAA24023..
408 -> 437 CIRSLEHAYSKDGGLAVLYGNFAENGCIVK UniProt: In Ref. 2; AAA24023..
610 -> 610 D UniProt: In Ref. 2; AAA24023..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.18 - isoleucine/valine
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0016836 - hydro-lyase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0051537 - 2 iron, 2 sulfur cluster binding
GO:0004160 - dihydroxy-acid dehydratase activity
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009082 - branched-chain amino acid biosynthetic process
GO:0009099 - valine biosynthetic process
GO:0009097 - isoleucine biosynthetic process
External database links:  
ALPHAFOLD:
 P05791
DIP:
 DIP-10021N
ECOCYC:
 DIHYDROXYACIDDEHYDRAT-MONOMER
ECOLIWIKI:
 b3771
INTERPRO:
 IPR042096
INTERPRO:
 IPR020558
INTERPRO:
 IPR004404
INTERPRO:
 IPR000581
INTERPRO:
 IPR037237
PFAM:
 PF00920
PRIDE:
 P05791
PRODB:
 PRO_000023004
PROSITE:
 PS00886
PROSITE:
 PS00887
REFSEQ:
 YP_026248
SMR:
 P05791
UNIPROT:
 P05791


Operon      
Name: ilvLXGMEDA         
Operon arrangement:
Transcription unit        Promoter
ilvL
ilvLXG_1G_2MEDA
ilvL
ilvLXG_1G_2MEDA
ilvEDA
ilvA


Transcriptional Regulation      
Display Regulation             
Activated by: IHF
Repressed by: IHF, Lrp


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4393 3953336 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4394 3953347 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4395 3954417 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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