RegulonDB RegulonDB 11.1: Gene Form
   

malI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

malX malI hdhA MalI CRP MalI CRP anti-terminator terminator malYp malYp malXp malXp TSS_1899 TSS_1899 malIp malIp hdhAp hdhAp

Gene      
Name: malI    Texpresso search in the literature
Synonym(s): ECK1615, EG10557, b1620
Genome position(nucleotides): 1698152 <-- 1699180
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.94
External database links:  
ASAP:
 ABE-0005421
CGSC:
 18181
ECHOBASE:
 EB0552
ECOLIHUB:
 malI
OU-MICROARRAY:
 b1620
STRING:
 511145.b1620
COLOMBOS: malI


Product      
Name: DNA-binding transcriptional repressor MalI
Synonym(s): MalI
Sequence: Get amino acid sequence Fasta Format
Regulator Family: GalR/LacI
Cellular location: cytosol
Molecular weight: 36.625
Isoelectric point: 6.956
Motif(s):
 
Type Positions Sequence Comment
7 -> 61 ITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALRG UniProt: HTH lacI-type.
8 -> 53 TIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRN
9 -> 28 IHDVALAAGVSVSTVSLVLS UniProt: H-T-H motif.
65 -> 341 GVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLIIPARLIAA
287 -> 297 FTDATPTTLDD UniProt: In Ref. 1 and 2..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
  3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0032993 - protein-DNA complex
molecular_function GO:0003677 - DNA binding
GO:0003700 - DNA-binding transcription factor activity
GO:0000976 - transcription cis-regulatory region binding
GO:0001217 - DNA-binding transcription repressor activity
biological_process GO:0006355 - regulation of transcription, DNA-templated
GO:0045892 - negative regulation of transcription, DNA-templated
Note(s): Note(s): ...[more].
Evidence: [COMP] Inferred by computational analysis
[COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
[EXP-IEP] Inferred from expression pattern
Reference(s): [1] Reidl J., et al., 1991
[2] Reidl J., et al., 1989
External database links:  
ALPHAFOLD:
 P18811
DIP:
 DIP-10143N
ECOCYC:
 PD00361
ECOLIWIKI:
 b1620
INTERPRO:
 IPR010982
INTERPRO:
 IPR028082
INTERPRO:
 IPR001761
INTERPRO:
 IPR000843
MODBASE:
 P18811
PANTHER:
 PTHR30146:SF3
PFAM:
 PF00532
PFAM:
 PF00356
PRIDE:
 P18811
PRODB:
 PRO_000023150
PROSITE:
 PS00356
PROSITE:
 PS50932
REFSEQ:
 NP_416137
SMART:
 SM00354
SMR:
 P18811
UNIPROT:
 P18811


Operon      
Name: malI         
Operon arrangement:
Transcription unit        Promoter
malI


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: CRP, MalI


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1899 1699241 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Reidl J., Boos W., 1991, The malX malY operon of Escherichia coli encodes a novel enzyme II of the phosphotransferase system recognizing glucose and maltose and an enzyme abolishing the endogenous induction of the maltose system., J Bacteriol 173(15):4862-76

 [2] Reidl J., Romisch K., Ehrmann M., Boos W., 1989, MalI, a novel protein involved in regulation of the maltose system of Escherichia coli, is highly homologous to the repressor proteins GalR, CytR, and LacI., J Bacteriol 171(9):4888-99

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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