RegulonDB RegulonDB 11.1: Gene Form
   

manA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fumA ydgA manA CRP CRP CRP CRP CRP CRP CRP MarA terminator terminator TSS_1897 TSS_1897 ydgAp ydgAp manAp manAp TSS_1895 TSS_1895 fumAp fumAp

Gene      
Name: manA    Texpresso search in the literature
Synonym(s): ECK1608, EG10566, b1613, pmi
Genome position(nucleotides): 1688576 --> 1689751
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.77
External database links:  
ASAP:
 ABE-0005398
CGSC:
 525
ECHOBASE:
 EB0561
ECOLIHUB:
 manA
MIM:
 602579
OU-MICROARRAY:
 b1613
STRING:
 511145.b1613
COLOMBOS: manA


Product      
Name: mannose-6-phosphate isomerase
Synonym(s): ManA, Pmi
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 42.85
Isoelectric point: 5.13
Motif(s):
 
Type Positions Sequence Comment
1 -> 150 MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKHNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFRE
157 -> 236 LLQPVAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLL
310 -> 357 AELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQ

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.2 - colanic acid (M antigen)
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.10 - sugar nucleotide biosynthesis, conversions
  5 - cell processes --> 5.5 - adaptations --> 5.5.5 - dessication
  6 - cell structure --> 6.7 - capsule (M and K antigens)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016853 - isomerase activity
GO:0046872 - metal ion binding
GO:0008270 - zinc ion binding
GO:0004476 - mannose-6-phosphate isomerase activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0009242 - colanic acid biosynthetic process
GO:0009298 - GDP-mannose biosynthetic process
GO:0019309 - mannose catabolic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P00946
DIP:
 DIP-10153N
ECOCYC:
 MANNPISOM-MONOMER
ECOLIWIKI:
 b1613
INTERPRO:
 IPR016305
INTERPRO:
 IPR018050
INTERPRO:
 IPR014710
INTERPRO:
 IPR011051
INTERPRO:
 IPR001250
MODBASE:
 P00946
PANTHER:
 PTHR10309
PFAM:
 PF01238
PFAM:
 PF20511
PFAM:
 PF20512
PRIDE:
 P00946
PRINTS:
 PR00714
PROSITE:
 PS00966
PROSITE:
 PS00965
REFSEQ:
 NP_416130
SMR:
 P00946
UNIPROT:
 P00946


Operon      
Name: manA         
Operon arrangement:
Transcription unit        Promoter
manA


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: CRP


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1895 1688441 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1897 1689797 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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