RegulonDB RegulonDB 11.1: Gene Form
   

manX gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

manX manY yoaL yoaE Cra NagC Mlc CRP Mlc NagC CRP Qin prophage; DicF SgrS TSS_2168 TSS_2168 TSS_2167 TSS_2167 TSS_2166 TSS_2166 TSS_2165 (cluster) TSS_2165 (cluster) TSS_2164 TSS_2164 TSS_2163 TSS_2163 TSS_2162 TSS_2162 TSS_2161 TSS_2161 TSS_2160 (cluster) TSS_2160 (cluster) TSS_2159 TSS_2159 TSS_2158 TSS_2158 TSS_2157 TSS_2157 TSS_2156 (cluster) TSS_2156 (cluster) TSS_2155 TSS_2155 TSS_2154 TSS_2154 TSS_2153 TSS_2153 TSS_2152 TSS_2152 TSS_2151 TSS_2151 TSS_2150 TSS_2150 TSS_2149 TSS_2149 TSS_2148 TSS_2148 TSS_2147 (cluster) TSS_2147 (cluster) TSS_2146 TSS_2146 TSS_2145 TSS_2145 TSS_2144 TSS_2144 TSS_2143 TSS_2143 TSS_2142 (cluster) TSS_2142 (cluster) manXp manXp yoaEp yoaEp TSS_2140 TSS_2140

Gene      
Name: manX    Texpresso search in the literature
Synonym(s): ECK1815, EG10567, b1817, gptB, mpt, ptsL, ptsX
Genome position(nucleotides): 1902048 --> 1903019
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.31
External database links:  
ASAP:
 ABE-0006054
CGSC:
 17656
ECHOBASE:
 EB0562
ECOLIHUB:
 manX
OU-MICROARRAY:
 b1817
STRING:
 511145.b1817
COLOMBOS: manX


Product      
Name: mannose-specific PTS enzyme IIAB component
Synonym(s): Enzyme IIABman, Enzyme IIIman, GptB, IIIMan, ManX, Mpt, PtsL, PtsM, PtsX
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 35.048
Isoelectric point: 5.817
Motif(s):
 
Type Positions Sequence Comment
2 -> 323 TIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPNDYMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK UniProt: PTS system mannose-specific EIIAB component.
5 -> 116 IVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVA
10 -> 10 H UniProt: Results in the formation of a single complex corresponding to the productive phosphoryl transfer complex..
12 -> 12 W UniProt: Slight phosphotransferase activity. Unable to dimerize..
35 -> 35 D UniProt: In Ref. 1; AAA24110..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  4 - transport --> 4.4 - Group Translocators --> 4.4.A - Phosphotransferase Systems (PEP-dependent PTS)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:1902495 - transmembrane transporter complex
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0016773 - phosphotransferase activity, alcohol group as acceptor
GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
GO:0042803 - protein homodimerization activity
GO:0022870 - protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity
biological_process GO:0098708 - glucose import across plasma membrane
GO:0008643 - carbohydrate transport
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system
GO:0016310 - phosphorylation
GO:0015764 - N-acetylglucosamine transport
GO:0015761 - mannose transmembrane transport
GO:0032445 - fructose import
Note(s): Note(s): ...[more].
Reference(s): [1] Nunn RS., et al., 1996
[2] Seip S., et al., 1997
External database links:  
ALPHAFOLD:
 P69797
DIP:
 DIP-35846N
ECOCYC:
 MANX-MONOMER
ECOLIWIKI:
 b1817
INTERPRO:
 IPR018455
INTERPRO:
 IPR033887
INTERPRO:
 IPR036662
INTERPRO:
 IPR004720
INTERPRO:
 IPR004701
INTERPRO:
 IPR036667
INTERPRO:
 IPR013789
MINT:
 P69797
PDB:
 2JZO
PDB:
 2JZN
PDB:
 2JZH
PDB:
 1VSQ
PDB:
 1PDO
PDB:
 1VRC
PFAM:
 PF03610
PFAM:
 PF03830
PRIDE:
 P69797
PRODB:
 PRO_000023161
PROSITE:
 PS51101
PROSITE:
 PS51096
REFSEQ:
 NP_416331
SMR:
 P69797
SWISSMODEL:
 P69797
UNIPROT:
 P69797


Operon      
Name: manXYZ         
Operon arrangement:
Transcription unit        Promoter
manXYZ


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: NagC, Mlc, Cra


Regulation by small RNA    
  Display Regulation
small RNA dicF, sgrS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2140 1901769 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2142 (cluster) 1901934 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2143 1902030 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2144 1902032 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2145 1902072 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2146 1902618 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2147 (cluster) 1903044 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2148 1903052 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2149 1903059 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2150 1903166 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2151 1903202 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2152 1903244 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2153 1903321 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2154 1903409 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2155 1903587 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2156 (cluster) 1903744 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2157 1903789 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2158 1903802 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2159 1903806 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2160 (cluster) 1903812 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2161 1903815 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2162 1903818 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2163 1903821 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2164 1903823 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2165 (cluster) 1903860 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2166 1903863 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2167 1903867 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2168 1903873 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Nunn RS., Markovic-Housley Z., Genovesio-Taverne JC., Flukiger K., Rizkallah PJ., Jansonius JN., Schirmer T., Erni B., 1996, Structure of the IIA domain of the mannose transporter from Escherichia coli at 1.7 angstroms resolution., J Mol Biol 259(3):502-11

 [2] Seip S., Lanz R., Gutknecht R., Flukiger K., Erni B., 1997, The fructose transporter of Bacillus subtilis encoded by the lev operon: backbone assignment and secondary structure of the IIB(Lev) subunit., Eur J Biochem 243(1-2):306-14

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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