RegulonDB RegulonDB 10.9: Gene Form
   

metA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

aceB metA yjaB IclR CRP IclR IclR IHF IclR IclR IclR IclR IHF Cra MetJ MetJ terminator TSS_4836 TSS_4836 TSS_4835 TSS_4835 aceBp aceBp metAp1 metAp1 metAp2 metAp2 yjaBp yjaBp

Gene      
Name: metA    Texpresso search in the literature
Synonym(s): ECK4005, EG10581, b4013
Genome position(nucleotides): 4214280 --> 4215209 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.0
External database links:  
ASAP:
ABE-0013121
CGSC:
516
ECHOBASE:
EB0576
ECOLIHUB:
metA
OU-MICROARRAY:
b4013
STRING:
511145.b4013
COLOMBOS: metA


Shine dalgarno      
Sequence: cgtatgtagtGAGGTAatcaggttATG


Product      
Name: homoserine O-succinyltransferase
Synonym(s): MetA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 35.727
Isoelectric point: 4.851
Motif(s):
 
Type Positions Sequence
46 -> 46 K
47 -> 47 K
90 -> 90 C
193 -> 193 R
246 -> 246 E

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.9 - methionine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0004414 - homoserine O-acetyltransferase activity
GO:0016740 - transferase activity
GO:0016746 - transferase activity, transferring acyl groups
GO:0008899 - homoserine O-succinyltransferase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009086 - methionine biosynthetic process
GO:0019281 - L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine
External database links:  
ECOCYC:
HOMSUCTRAN-MONOMER
ECOLIWIKI:
b4013
INTERPRO:
IPR005697
INTERPRO:
IPR033752
INTERPRO:
IPR029062
MINT:
P07623
MODBASE:
P07623
PANTHER:
PTHR20919
PDB:
6MTG
PFAM:
PF04204
PRIDE:
P07623
PRODB:
PRO_000023208
REFSEQ:
NP_418437
SMR:
P07623
SWISSMODEL:
P07623
UNIPROT:
P07623


Operon      
Name: metA         
Operon arrangement:
Transcription unit        Promoter
metA
metA


Transcriptional Regulation      
Display Regulation             
Repressed by: MetJ
Growth Conditions:

[1] 

C: Escherichia coli| minimal medium| amino acids 50 µg; glucose 0.2%; thiamine 1 µg/mL| 32.0 C| exponential phase
E: Escherichia coli| minimal medium| amino acids 50 µg; glucose 0.2%; methionine 200 µg/mL; thiamine 1 µg/mL| 32.0 C| exponential phase

[2] 

C: Escherichia coli| MOPS| amino acids 50 µg; glucose 0.2%; thiamine 1 µg/mL| 32.0 C| exponential phase
E: Escherichia coli| MOPS| amino acids 50 µg; cadmium dichloride 600 µM; glucose 0.2%; thiamine 1 µg/mL| 32.0 C| exponential phase



Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yjaBp 4214156 reverse The promoter of the yjaB
Read more >
[AIPP], [ICWHO] [1], [2]
  promoter TSS_4835 4215404 forward nd [RS-EPT-CBR] [3]
  promoter TSS_4836 4217043 forward nd [RS-EPT-CBR] [3]


Evidence    

 [AIPP] Automated inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Gery S., Ron EZ., 1997, An Escherichia coli gene divergently transcribed from a promoter overlapping the metA promoter., FEMS Microbiol Lett 154(2):219-22

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muńiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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