RegulonDB RegulonDB 10.10: Gene Form
   

metC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

metC exbB yghB unknown Fur Fur Fur TSS_3317 TSS_3317 yghBp yghBp metCp metCp exbBp exbBp exbDp exbDp

Gene      
Name: metC    Texpresso search in the literature
Synonym(s): ECK3000, EG10583, b3008
Genome position(nucleotides): 3152236 --> 3153423 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.01
External database links:  
ASAP:
 ABE-0009878
CGSC:
 514
ECHOBASE:
 EB0578
ECOLIHUB:
 metC
NCBI-GENE:
 946240
OU-MICROARRAY:
 b3008
STRING:
 511145.b3008
COLOMBOS: metC


Shine dalgarno      
Sequence: gtataaaaacAGGAATcccgacATG


Product      
Name: cystathionine β-lyase / L-cysteine desulfhydrase / alanine racemase
Synonym(s): MetC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 43.212
Isoelectric point: 6.44
Motif(s):
 
Type Positions Sequence
7 -> 391 DTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGF
1 -> 1 M
2 -> 395 ADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFARIV

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.9 - methionine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0032991 - protein-containing complex
molecular_function GO:0047804 - cysteine-S-conjugate beta-lyase activity
GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0030170 - pyridoxal phosphate binding
GO:0004121 - cystathionine beta-lyase activity
GO:0008784 - alanine racemase activity
GO:0042802 - identical protein binding
GO:0080146 - L-cysteine desulfhydrase activity
biological_process GO:0006520 - cellular amino acid metabolic process
GO:0008652 - cellular amino acid biosynthetic process
GO:0009086 - methionine biosynthetic process
GO:0051289 - protein homotetramerization
GO:0019346 - transsulfuration
GO:0019450 - L-cysteine catabolic process to pyruvate
External database links:  
DIP:
 DIP-10193N
ECOCYC:
 CYSTATHIONINE-BETA-LYASE-MONOMER
ECOLIWIKI:
 b3008
INTERPRO:
 IPR000277
INTERPRO:
 IPR015424
INTERPRO:
 IPR006233
INTERPRO:
 IPR015421
INTERPRO:
 IPR015422
MODBASE:
 P06721
PANTHER:
 PTHR43500
PDB:
 1CL2
PDB:
 4ITX
PDB:
 4ITG
PDB:
 2GQN
PDB:
 2FQ6
PDB:
 1CL1
PFAM:
 PF01053
PRIDE:
 P06721
PRODB:
 PRO_000023210
PROSITE:
 PS00868
REFSEQ:
 NP_417481
SMR:
 P06721
UNIPROT:
 P06721


Operon      
Name: metC         
Operon arrangement:
Transcription unit        Promoter
metC


Transcriptional Regulation      
Display Regulation             
Repressed by: MetJ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3317 3153632 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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