RegulonDB RegulonDB 10.10: Gene Form
   

metE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

metE metR ysgA bioP MetJ MetJ OxyR MetR OxyR MetR TSS_4502 TSS_4502 TSS_4501 TSS_4501 TSS_4500 TSS_4500 TSS_4499 TSS_4499 TSS_4498 TSS_4498 TSS_4497 TSS_4497 TSS_4496 TSS_4496 TSS_4495 TSS_4495 TSS_4494 TSS_4494 TSS_4493 TSS_4493 TSS_4492 TSS_4492 TSS_4491 TSS_4491 TSS_4490 TSS_4490 TSS_4489 TSS_4489 TSS_4488 TSS_4488 TSS_4487 TSS_4487 TSS_4486 TSS_4486 TSS_4485 TSS_4485 TSS_4484 TSS_4484 metEp metEp metRp1 metRp1 metRp2 metRp2

Gene      
Name: metE    Texpresso search in the literature
Synonym(s): ECK3823, EG10584, b3829, metB12
Genome position(nucleotides): 4013053 --> 4015314 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.23
External database links:  
ASAP:
 ABE-0012520
CGSC:
 512
ECHOBASE:
 EB0579
ECOLIHUB:
 metE
OU-MICROARRAY:
 b3829
STRING:
 511145.b3829
COLOMBOS: metE


Product      
Name: cobalamin-independent homocysteine transmethylase
Synonym(s): MetE
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 84.673
Isoelectric point: 5.741
Motif(s):
 
Type Positions Sequence
643 -> 643 C
659 -> 659 A
5 -> 312 NHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPVLLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIV
2 -> 753 TILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELRARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRGRAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPVLLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAELHKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSVETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAVEKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKGNLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEAFRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDYPNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAALANMVQAAQNLRRG
363 -> 363 L

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.9 - methionine
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0008168 - methyltransferase activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0008270 - zinc ion binding
GO:0003871 - 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
GO:0008705 - methionine synthase activity
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009086 - methionine biosynthetic process
GO:0032259 - methylation
GO:0035999 - tetrahydrofolate interconversion
GO:0050667 - homocysteine metabolic process
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-6847N
ECOCYC:
 HOMOCYSMET-MONOMER
ECOLIWIKI:
 b3829
INTERPRO:
 IPR038071
INTERPRO:
 IPR013215
INTERPRO:
 IPR006276
INTERPRO:
 IPR002629
PFAM:
 PF01717
PFAM:
 PF08267
PRIDE:
 P25665
PRODB:
 PRO_000023211
REFSEQ:
 NP_418273
SMR:
 P25665
SWISSMODEL:
 P25665
UNIPROT:
 P25665


Operon      
Name: metE         
Operon arrangement:
Transcription unit        Promoter
metE


Transcriptional Regulation      
Display Regulation             
Activated by: MetR, OxyR
Repressed by: MetJ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4484 4012886 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4485 4012922 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4486 4012947 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4487 4013010 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4488 4013017 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4489 4013034 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4490 4013061 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4491 4013079 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4492 4013127 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4493 4013584 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4494 4013877 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4495 4013894 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4496 4013905 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4497 4013974 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4498 4014376 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4499 4014407 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4500 4014445 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4501 4014468 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4502 4015162 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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