RegulonDB

Gene
Name:	motB
Synonym(s):	ECK1890, EG10602, b1889, flaJ
Genome position(nucleotides):	1975329 <-- 1976255
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.18
External database links:

ASAP:	ABE-0006297
CGSC:	490
ECHOBASE:	EB0597
ECOLIHUB:	motB
OU-MICROARRAY:	b1889
STRING:	511145.b1889
COLOMBOS:	motB

Gene

Name:

motB

Synonym(s):

ECK1890, EG10602, b1889, flaJ

Genome position(nucleotides):

1975329 <-- 1976255

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

53.18

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

13 -> 66

RRKAKSHGAAHGSWKIAYADFMTAMMAFFLVMWLISISSPKELIQIAEYFRTPL

28 -> 49

IAYADFMTAMMAFFLVMWLISI

UniProt: Helical; Signal-anchor for type II membrane protein.

31 -> 31

UniProt: Complete loss of motility..

32 -> 32

UniProt: Complete loss of motility; see further |CITS: [9573160][11669642]|

39 -> 39

UniProt: Complete loss of motility..

Type

Positions

Sequence

Comment

13 -> 66

RRKAKSHGAAHGSWKIAYADFMTAMMAFFLVMWLISISSPKELIQIAEYFRTPL

28 -> 49

IAYADFMTAMMAFFLVMWLISI

UniProt: Helical; Signal-anchor for type II membrane protein.

31 -> 31

UniProt: Complete loss of motility..

32 -> 32

UniProt: Complete loss of motility; see further |CITS: [9573160][11669642]|

39 -> 39

UniProt: Complete loss of motility..

73 -> 101

GDRISNSESPIPGGGDDYTQSQGEVNKQP

UniProt: Disordered; Sequence Annotation Type: region of interest.

148 -> 268

LRIQIIDSQNRPMFRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRANASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQ

UniProt: OmpA-like.

159 -> 159

UniProt: Decreased motility, subnormal torque, tethered strains rotate very slowly..

160 -> 257

MFRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRANASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVN

164 -> 164

UniProt: Complete loss of motility..

196 -> 196

UniProt: Complete loss of motility..

197 -> 197

UniProt: Complete loss of motility..

205 -> 205

UniProt: Decreased motility, subnormal torque, tethered strains rotate very slowly, maybe reduced affinity for the motor..

210 -> 227

NWELSADRANASRRELMV

predicted peptidoglycan-associating α-helix

214 -> 214

UniProt: Complete loss of motility..

217 -> 217

UniProt: Complete loss of motility..

222 -> 222

UniProt: Complete loss of motility..

240 -> 240

UniProt: Decreased motility, subnormal torque, tethered strains rotate very slowly..

242 -> 242

UniProt: Complete loss of motility..

258 -> 258

UniProt: Complete loss of motility..

277 -> 308

NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR

UniProt: Disordered; Sequence Annotation Type: region of interest.

Multifun Terms (GenProtEC)

5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)

6 - cell structure --> 6.1 - membrane

6 - cell structure --> 6.4 - flagella

Gene Ontology Terms (GO)

cellular_component

GO:0120101 - bacterial-type flagellum stator complex
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0009288 - bacterial-type flagellum

molecular_function

GO:0015252 - proton channel activity
GO:0005515 - protein binding
GO:0042834 - peptidoglycan binding

biological_process

GO:0006935 - chemotaxis
GO:0071973 - bacterial-type flagellum-dependent cell motility
GO:1902600 - proton transmembrane transport
GO:0097588 - archaeal or bacterial-type flagellum-dependent cell motility

Transcriptional Regulation
	Display Regulation
Repressed by:	CpxR

Transcriptional Regulation

Display Regulation

Repressed by:

CpxR

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	cheWp5	1973462	reverse	nd	[COMP-AINF]	[15]
promoter	TSS_2228	1973726	reverse	nd	[RS-EPT-CBR]	[16]
promoter	TSS_2229	1975808	reverse	nd	[RS-EPT-CBR]	[16]
promoter	TSS_2230	1976720	reverse	nd	[RS-EPT-CBR]	[16]
promoter	TSS_2231	1977300	reverse	nd	[RS-EPT-CBR]	[16]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

cheWp5

1973462

reverse

[COMP-AINF]

[15]

promoter

TSS_2228

1973726

reverse

[RS-EPT-CBR]

[16]

promoter

TSS_2229

1975808

reverse

[RS-EPT-CBR]

[16]

promoter

TSS_2230

1976720

reverse

[RS-EPT-CBR]

[16]

promoter

TSS_2231

1977300

reverse

[RS-EPT-CBR]

[16]

Evidence
[COMP-AINF] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[COMP-AINF] Inferred computationally without human oversight

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Asai Y., Yakushi T., Kawagishi I., Homma M., 2003, Ion-coupling determinants of Na+-driven and H+-driven flagellar motors., J Mol Biol 327(2):453-63
[2] Berry RM., 2000, Theories of rotary motors., Philos Trans R Soc Lond B Biol Sci 355(1396):503-9
[3] Blair DF., Kim DY., Berg HC., 1991, Mutant MotB proteins in Escherichia coli., J Bacteriol 173(13):4049-55
[4] Block SM., Berg HC., 1984, Successive incorporation of force-generating units in the bacterial rotary motor., Nature 309(5967):470-2
[5] Boyd A., Mandel G., Simon MI., 1982, Integral membrane proteins required for bacterial motility and chemotaxis., Symp Soc Exp Biol 35:123-37
[6] Braun TF., Blair DF., 2001, Targeted disulfide cross-linking of the MotB protein of Escherichia coli: evidence for two H(+) channels in the stator Complex., Biochemistry 40(43):13051-9
[7] De Mot R., Vanderleyden J., 1994, The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan., Mol Microbiol 12(2):333-4
[8] Gosink KK., Hase CC., 2000, Requirements for conversion of the Na(+)-driven flagellar motor of Vibrio cholerae to the H(+)-driven motor of Escherichia coli., J Bacteriol 182(15):4234-40
[9] Ishida T., Ito R., Clark J., Matzke NJ., Sowa Y., Baker MAB., 2019, Sodium-powered stators of the bacterial flagellar motor can generate torque in the presence of phenamil with mutations near the peptidoglycan-binding region., Mol Microbiol 111(6):1689-1699
[10] Kojima S., Blair DF., 2001, Conformational change in the stator of the bacterial flagellar motor., Biochemistry 40(43):13041-50
[11] Minamino T., Terahara N., Kojima S., Namba K., 2018, Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment., Mol Microbiol 109(6):723-734
[12] Onoue Y., Takekawa N., Nishikino T., Kojima S., Homma M., 2018, The role of conserved charged residues in the bidirectional rotation of the bacterial flagellar motor., Microbiologyopen 7(4):e00587
[13] Sowa Y., Homma M., Ishijima A., Berry RM., 2014, Hybrid-fuel bacterial flagellar motors in Escherichia coli., Proc Natl Acad Sci U S A 111(9):3436-41
[14] Van Way SM., Hosking ER., Braun TF., Manson MD., 2000, Mot protein assembly into the bacterial flagellum: a model based on mutational analysis of the motB gene., J Mol Biol 297(1):7-24
[15] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[16] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Asai Y., Yakushi T., Kawagishi I., Homma M., 2003, Ion-coupling determinants of Na+-driven and H+-driven flagellar motors., J Mol Biol 327(2):453-63

[2] Berry RM., 2000, Theories of rotary motors., Philos Trans R Soc Lond B Biol Sci 355(1396):503-9

[3] Blair DF., Kim DY., Berg HC., 1991, Mutant MotB proteins in Escherichia coli., J Bacteriol 173(13):4049-55

[4] Block SM., Berg HC., 1984, Successive incorporation of force-generating units in the bacterial rotary motor., Nature 309(5967):470-2

[5] Boyd A., Mandel G., Simon MI., 1982, Integral membrane proteins required for bacterial motility and chemotaxis., Symp Soc Exp Biol 35:123-37

[6] Braun TF., Blair DF., 2001, Targeted disulfide cross-linking of the MotB protein of Escherichia coli: evidence for two H(+) channels in the stator Complex., Biochemistry 40(43):13051-9

[7] De Mot R., Vanderleyden J., 1994, The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both gram-positive and gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan., Mol Microbiol 12(2):333-4

[8] Gosink KK., Hase CC., 2000, Requirements for conversion of the Na(+)-driven flagellar motor of Vibrio cholerae to the H(+)-driven motor of Escherichia coli., J Bacteriol 182(15):4234-40

[9] Ishida T., Ito R., Clark J., Matzke NJ., Sowa Y., Baker MAB., 2019, Sodium-powered stators of the bacterial flagellar motor can generate torque in the presence of phenamil with mutations near the peptidoglycan-binding region., Mol Microbiol 111(6):1689-1699

[10] Kojima S., Blair DF., 2001, Conformational change in the stator of the bacterial flagellar motor., Biochemistry 40(43):13041-50

[11] Minamino T., Terahara N., Kojima S., Namba K., 2018, Autonomous control mechanism of stator assembly in the bacterial flagellar motor in response to changes in the environment., Mol Microbiol 109(6):723-734

[12] Onoue Y., Takekawa N., Nishikino T., Kojima S., Homma M., 2018, The role of conserved charged residues in the bidirectional rotation of the bacterial flagellar motor., Microbiologyopen 7(4):e00587

[13] Sowa Y., Homma M., Ishijima A., Berry RM., 2014, Hybrid-fuel bacterial flagellar motors in Escherichia coli., Proc Natl Acad Sci U S A 111(9):3436-41

[14] Van Way SM., Hosking ER., Braun TF., Manson MD., 2000, Mot protein assembly into the bacterial flagellum: a model based on mutational analysis of the motB gene., J Mol Biol 297(1):7-24

[15] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

[16] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.