RegulonDB RegulonDB 11.0: Gene Form
   

pcm gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nlpD truD umpG pcm ispF MqsA ArcA CRP ArcA CRP DksA-ppGpp DksA-ppGpp ppGpp surEp2 surEp2 nlpDp1 nlpDp1 nlpDp2 nlpDp2 TSS_3034 TSS_3034 rpoSp rpoSp rpoSp4 rpoSp4 rpoSp3 rpoSp3 TSS_3032 TSS_3032

Gene      
Name: pcm    Texpresso search in the literature
Synonym(s): ECK2738, EG10689, b2743
Genome position(nucleotides): 2868893 <-- 2869519
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.43
External database links:  
ASAP:
ABE-0009006
CGSC:
33221
ECHOBASE:
EB0683
ECOLIHUB:
pcm
OU-MICROARRAY:
b2743
STRING:
511145.b2743
COLOMBOS: pcm


Product      
Name: protein-L-isoaspartate O-methyltransferase
Synonym(s): L-isoaspartate protein carboxylmethyltransferase type II, Pcm
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 23.258
Isoelectric point: 7.073
Motif(s):
 
Type Positions Sequence Comment
2 -> 208 VSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMTQLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA UniProt: Protein-L-isoaspartate O-methyltransferase.
5 -> 206 RVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKAWDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCSVERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMTQLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGE

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0008168 - methyltransferase activity
GO:0016740 - transferase activity
GO:0004719 - protein-L-isoaspartate (D-aspartate) O-methyltransferase activity
biological_process GO:0006464 - cellular protein modification process
GO:0006479 - protein methylation
GO:0030091 - protein repair
GO:0032259 - methylation
Note(s): Note(s): ...[more].
Reference(s): [1] Brennan TV., et al., 1994
[2] David CL., et al., 1999
[3] Kagan RM., et al., 1997
[4] Sadakane Y., et al., 2020
External database links:  
ALPHAFOLD:
P0A7A5
ECOCYC:
EG10689-MONOMER
ECOLIWIKI:
b2743
INTERPRO:
IPR000682
INTERPRO:
IPR029063
MODBASE:
P0A7A5
PANTHER:
PTHR11579
PDB:
3LBF
PFAM:
PF01135
PRIDE:
P0A7A5
PRODB:
PRO_000023499
PROSITE:
PS01279
REFSEQ:
NP_417223
SMR:
P0A7A5
SWISSMODEL:
P0A7A5
UNIPROT:
P0A7A5


Operon      
Name: umpG-pcm         
Operon arrangement:
Transcription unit        Promoter
umpG-pcm


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3032 2867645 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_3034 2868128 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Brennan TV., Anderson JW., Jia Z., Waygood EB., Clarke S., 1994, Repair of spontaneously deamidated HPr phosphocarrier protein catalyzed by the L-isoaspartate-(D-aspartate) O-methyltransferase., J Biol Chem 269(40):24586-95

 [2] David CL., Keener J., Aswad DW., 1999, Isoaspartate in ribosomal protein S11 of Escherichia coli., J Bacteriol 181(9):2872-7

 [3] Kagan RM., McFadden HJ., McFadden PN., O'Connor C., Clarke S., 1997, Molecular phylogenetics of a protein repair methyltransferase., Comp Biochem Physiol B Biochem Mol Biol 117(3):379-85

 [4] Sadakane Y., Senda S., Deguchi T., Tanaka A., Tsuruta H., Morimoto S., 2020, Effect of amino acids present at the carboxyl end of succinimidyl residue on the rate constants for succinimidyl hydrolysis in small peptides., Biochim Biophys Acta Proteins Proteom 1868(11):140496

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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