RegulonDB RegulonDB 11.1: Gene Form
   

plsB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

plsB ubiA dgkA BasR anti-terminator terminator dgkAp2 dgkAp2 plsBp1 plsBp1 plsBp2 plsBp2 dgkAp1 dgkAp1 TSS_4879 TSS_4879 TSS_4878 TSS_4878 TSS_4877 TSS_4877

Gene      
Name: plsB    Texpresso search in the literature
Synonym(s): ECK4033, EG10740, b4041
Genome position(nucleotides): 4254043 <-- 4256466
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.16
Reference(s): [1] Lightner VA., et al., 1983
External database links:  
ASAP:
 ABE-0013232
CGSC:
 382
ECHOBASE:
 EB0733
ECOLIHUB:
 plsB
MIM:
 222765
OU-MICROARRAY:
 b4041
STRING:
 511145.b4041
COLOMBOS: plsB


Product      
Name: glycerol-3-phosphate 1-O-acyltransferase
Synonym(s): sn-glycerol-3-phosphate acyltransferase, GPAT, PlsB, glycerol-3-phosphate O-acyltransferase, glycerolphosphate acyltransferase
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 91.381
Isoelectric point: 8.565
Motif(s):
 
Type Positions Sequence Comment
2 -> 807 SGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIESATQGEG UniProt: Glycerol-3-phosphate acyltransferase.
282 -> 424 INVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYI
306 -> 306 H UniProt: Abolishes acyltransferase activity..
308 -> 308 S UniProt: No effect..
311 -> 311 D UniProt: Prevents assembly into the membrane, suggesting that it participates in folding..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.4 - fatty acids and phosphatidic acid
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.1 - phospholipid
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
molecular_function GO:0102420 - sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0016746 - acyltransferase activity
GO:0008374 - O-acyltransferase activity
GO:0004366 - glycerol-3-phosphate O-acyltransferase activity
biological_process GO:0019432 - triglyceride biosynthetic process
GO:0006629 - lipid metabolic process
GO:0006631 - fatty acid metabolic process
GO:0044255 - cellular lipid metabolic process
GO:0008654 - phospholipid biosynthetic process
GO:0006072 - glycerol-3-phosphate metabolic process
GO:0016024 - CDP-diacylglycerol biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0A7A7
DIP:
 DIP-29380N
ECOCYC:
 GLYCEROL-3-P-ACYLTRANSFER-MONOMER
ECOLIWIKI:
 b4041
INTERPRO:
 IPR041728
INTERPRO:
 IPR028354
INTERPRO:
 IPR022284
INTERPRO:
 IPR002123
PANTHER:
 PTHR12563
PFAM:
 PF01553
PFAM:
 PF19277
PRIDE:
 P0A7A7
REFSEQ:
 NP_418465
SMART:
 SM00563
UNIPROT:
 P0A7A7


Operon      
Name: plsB         
Operon arrangement:
Transcription unit        Promoter
plsB
plsB


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4877 4255382 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4878 4255992 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4879 4256011 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Lightner VA., Bell RM., Modrich P., 1983, The DNA sequences encoding plsB and dgk loci of Escherichia coli., J Biol Chem 258(18):10856-61

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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