RegulonDB RegulonDB 10.10: Gene Form
   

potE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

speF potE ybfG ybfP ybfK ybfI speFL potEp1 potEp1 ybfHp ybfHp

Gene      
Name: potE    Texpresso search in the literature
Synonym(s): ECK0680, EG10753, b0692
Genome position(nucleotides): 716946 <-- 718265 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.61
External database links:  
ASAP:
 ABE-0002360
CGSC:
 31552
ECHOBASE:
 EB0746
ECOLIHUB:
 potE
OU-MICROARRAY:
 b0692
STRING:
 511145.b0692
COLOMBOS: potE


Product      
Name: putrescine transporter PotE
Synonym(s): PotE, putrescine symporter, putrescine:ornithine antiporter
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 46.495
Isoelectric point: 8.536
Motif(s):
 
Type Positions Sequence
82 -> 82 K
40 -> 60 ISIISWLVTAVGSMALAWAFA
152 -> 172 ITVWGVIIPVVGLCIIGWFWF
210 -> 210 C
276 -> 296 VIMALMVMSCCGSLLGWQFTI

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.4 - amines
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015297 - antiporter activity
GO:0015293 - symporter activity
GO:0015489 - putrescine transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0015496 - putrescine:ornithine antiporter activity
biological_process GO:0006865 - amino acid transport
GO:0055085 - transmembrane transport
GO:0015847 - putrescine transport
GO:0015822 - ornithine transport
GO:1903352 - L-ornithine transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Jindal S., et al., 2019
[2] Kashiwagi K., et al., 1990
[3] Kashiwagi K., et al., 1986
[4] Kashiwagi K., et al., 2000
[5] Minchin RF., et al., 2004
[6] Munro GF., et al., 1974
[7] Tomitori H., et al., 2012
External database links:  
ECOCYC:
 POTE-MONOMER
ECOLIWIKI:
 b0692
INTERPRO:
 IPR004754
INTERPRO:
 IPR027566
INTERPRO:
 IPR002293
PFAM:
 PF13520
PRIDE:
 P0AAF1
PRODB:
 PRO_000023571
REFSEQ:
 NP_415219
UNIPROT:
 P0AAF1


Operon      
Name: speFLF-potE         
Operon arrangement:
Transcription unit        Promoter
speF-potE


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter potEp1 718344 reverse nd [ICWHO] [8]


Evidence    

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [2] Kashiwagi K., Hosokawa N., Furuchi T., Kobayashi H., Sasakawa C., Yoshikawa M., Igarashi K., 1990, Isolation of polyamine transport-deficient mutants of Escherichia coli and cloning of the genes for polyamine transport proteins., J Biol Chem 265(34):20893-7

 [3] Kashiwagi K., Kobayashi H., Igarashi K., 1986, Apparently unidirectional polyamine transport by proton motive force in polyamine-deficient Escherichia coli., J Bacteriol 165(3):972-7

 [4] Kashiwagi K., Kuraishi A., Tomitori H., Igarashi A., Nishimura K., Shirahata A., Igarashi K., 2000, Identification of the putrescine recognition site on polyamine transport protein PotE., J Biol Chem 275(46):36007-12

 [5] Minchin RF., McCoubrie JE., 2004, Mutational analysis of the large periplasmic loop 7-8 of the putrescine transporter PotE in Escherichia coli., Int J Biochem Cell Biol 36(2):271-80

 [6] Munro GF., Bell CA., Lederman M., 1974, Multiple transport components for putrescine in Escherichia coli., J Bacteriol 118(3):952-63

 [7] Tomitori H., Kashiwagi K., Igarashi K., 2012, Structure and function of polyamine-amino acid antiporters CadB and PotE in Escherichia coli., Amino Acids 42(2-3):733-40

 [8] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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