RegulonDB RegulonDB 10.10: Gene Form
   

secY gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

secY rplO rpmJ DksA-ppGpp DksA-ppGpp anti-anti-terminator TSS_3727 TSS_3727 TSS_3726 TSS_3726 TSS_3725 TSS_3725 TSS_3724 TSS_3724 TSS_3723 TSS_3723 TSS_3722 TSS_3722 TSS_3721 TSS_3721 TSS_3720 (cluster) TSS_3720 (cluster) TSS_3719 TSS_3719 TSS_3718 (cluster) TSS_3718 (cluster) TSS_3717 TSS_3717 TSS_3716 TSS_3716 TSS_3715 (cluster) TSS_3715 (cluster) TSS_3714 TSS_3714 TSS_3713 TSS_3713 TSS_3712 TSS_3712 TSS_3711 TSS_3711 TSS_3710 TSS_3710 TSS_3709 (cluster) TSS_3709 (cluster) TSS_3708 TSS_3708 TSS_3707 TSS_3707 TSS_3706 (cluster) TSS_3706 (cluster) TSS_3705 (cluster) TSS_3705 (cluster) TSS_3704 TSS_3704 TSS_3703 TSS_3703 TSS_3702 (cluster) TSS_3702 (cluster) TSS_3701 TSS_3701 TSS_3700 TSS_3700 TSS_3699 TSS_3699 TSS_3698 (cluster) TSS_3698 (cluster) TSS_3697 TSS_3697 TSS_3696 TSS_3696 TSS_3695 (cluster) TSS_3695 (cluster) TSS_3694 (cluster) TSS_3694 (cluster) TSS_3693 TSS_3693 TSS_3692 TSS_3692 TSS_3691 (cluster) TSS_3691 (cluster) TSS_3690 TSS_3690 TSS_3689 TSS_3689 TSS_3688 TSS_3688 TSS_3687 (cluster) TSS_3687 (cluster) TSS_3686 TSS_3686 TSS_3685 (cluster) TSS_3685 (cluster) TSS_3684 (cluster) TSS_3684 (cluster) TSS_3683 TSS_3683 TSS_3682 TSS_3682 TSS_3681 (cluster) TSS_3681 (cluster) TSS_3680 TSS_3680 TSS_3679 (cluster) TSS_3679 (cluster) TSS_3678 TSS_3678 TSS_3677 (cluster) TSS_3677 (cluster)

Gene      
Name: secY    Texpresso search in the literature
Synonym(s): ECK3287, EG10766, b3300, prlA
Genome position(nucleotides): 3442766 <-- 3444097 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.7
External database links:  
ASAP:
 ABE-0010814
CGSC:
 18037
ECHOBASE:
 EB0759
ECOLIHUB:
 secY
OU-MICROARRAY:
 b3300
STRING:
 511145.b3300
COLOMBOS: secY


Product      
Name: Sec translocon subunit SecY
Synonym(s): PrlA, SecY
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 48.512
Isoelectric point: 10.335
Motif(s):
 
Type Positions Sequence
165 -> 178 VTGTMFLMWLGEQI
224 -> 237 AVLVFAVTFFVVFV
287 -> 287 P
381 -> 395 ITFICLIPEFMRDAM
67 -> 67 F

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.5 - The Type II (General) Secretory Pathway (IISP) Family
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0031522 - cell envelope Sec protein transport complex
molecular_function GO:1901612 - cardiolipin binding
GO:0005515 - protein binding
GO:0005048 - signal sequence binding
GO:0008320 - protein transmembrane transporter activity
biological_process GO:0015031 - protein transport
GO:0006605 - protein targeting
GO:0006886 - intracellular protein transport
GO:0065002 - intracellular protein transmembrane transport
GO:0032978 - protein insertion into membrane from inner side
GO:0043952 - protein transport by the Sec complex
GO:0006616 - SRP-dependent cotranslational protein targeting to membrane, translocation
Note(s): Note(s): ...[more].
Reference(s): [1] Akiyama Y., et al., 1989
[2] Baba T., et al., 1994
[3] Fandl JP., et al., 1988
[4] Flower AM., et al., 1994
[5] Kuhn P., et al., 2015
[6] Satoh Y., et al., 2003
[7] Shimoike T., et al., 1992
[8] Shimokawa N., et al., 2003
[9] Taura T., et al., 1994
[10] Taura T., et al., 1997
[11] Veenendaal AK., et al., 2001
[12] Watanabe M., et al., 1989
[13] van der Sluis EO., et al., 2002
External database links:  
DIP:
 DIP-59302N
ECOCYC:
 SECY
ECOLIWIKI:
 b3300
INTERPRO:
 IPR002208
INTERPRO:
 IPR023201
INTERPRO:
 IPR026593
INTERPRO:
 IPR030659
MODBASE:
 P0AGA2
PANTHER:
 PTHR10906
PDB:
 5GAE
PDB:
 4V6M
PDB:
 6R7L
PDB:
 5NCO
PDB:
 5MG3
PDB:
 5ABB
PDB:
 2AKH
PDB:
 2AKI
PDB:
 3J45
PDB:
 3J46
PFAM:
 PF00344
PRIDE:
 P0AGA2
PRODB:
 PRO_000023930
PROSITE:
 PS00756
PROSITE:
 PS00755
REFSEQ:
 NP_417759
SMR:
 P0AGA2
UNIPROT:
 P0AGA2


Operon      
Name: rplNXE-rpsNH-rplFR-rpsE-rpmD-rplO-secY-rpmJ         
Operon arrangement:
Transcription unit        Promoter
rplNXE-rpsNH-rplFR-rpsE-rpmD-rplO-secY-rpmJ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3677 (cluster) 3442845 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3678 3442853 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3679 (cluster) 3442861 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3680 3442874 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3681 (cluster) 3442902 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3682 3442924 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3683 3442927 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3684 (cluster) 3442931 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3685 (cluster) 3442939 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3686 3442946 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3687 (cluster) 3442950 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3688 3442952 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3689 3443130 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3690 3443181 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3691 (cluster) 3443248 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3692 3443251 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3693 3443264 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3694 (cluster) 3443307 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3695 (cluster) 3443351 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3696 3443608 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3697 3443611 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3698 (cluster) 3443618 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3699 3443627 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3700 3443728 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3701 3443964 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3702 (cluster) 3443970 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3703 3443983 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3704 3444065 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3705 (cluster) 3444077 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3706 (cluster) 3444080 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3707 3444086 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3708 3444089 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3709 (cluster) 3444093 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3710 3444097 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3711 3444116 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3712 3444233 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3713 3444262 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3714 3444279 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3715 (cluster) 3444289 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3716 3444332 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3717 3444337 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3718 (cluster) 3444340 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3719 3444345 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3720 (cluster) 3444352 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3721 3444474 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3722 3444481 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3723 3444485 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3724 3444497 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3725 3444499 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3726 3444507 reverse nd [RS-EPT-CBR] [14]
  promoter TSS_3727 3444510 reverse nd [RS-EPT-CBR] [14]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Akiyama Y., Ito K., 1989, Export of Escherichia coli alkaline phosphatase attached to an integral membrane protein, SecY., J Biol Chem 264(1):437-42

 [2] Baba T., Taura T., Shimoike T., Akiyama Y., Yoshihisa T., Ito K., 1994, A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex., Proc Natl Acad Sci U S A 91(10):4539-43

 [3] Fandl JP., Cabelli R., Oliver D., Tai PC., 1988, SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles., Proc Natl Acad Sci U S A 85(23):8953-7

 [4] Flower AM., Doebele RC., Silhavy TJ., 1994, PrlA and PrlG suppressors reduce the requirement for signal sequence recognition., J Bacteriol 176(18):5607-14

 [5] Kuhn P., Draycheva A., Vogt A., Petriman NA., Sturm L., Drepper F., Warscheid B., Wintermeyer W., Koch HG., 2015, Ribosome binding induces repositioning of the signal recognition particle receptor on the translocon., J Cell Biol 211(1):91-104

 [6] Satoh Y., Mori H., Ito K., 2003, Nearest neighbor analysis of the SecYEG complex. 2. Identification of a SecY-SecE cytosolic interface., Biochemistry 42(24):7442-7

 [7] Shimoike T., Akiyama Y., Baba T., Taura T., Ito K., 1992, SecY variants that interfere with Escherichia coli protein export in the presence of normal secY., Mol Microbiol 6(9):1205-10

 [8] Shimokawa N., Mori H., Ito K., 2003, Importance of transmembrane segments in Escherichia coli SecY., Mol Genet Genomics 269(2):180-7

 [9] Taura T., Akiyama Y., Ito K., 1994, Genetic analysis of SecY: additional export-defective mutations and factors affecting their phenotypes., Mol Gen Genet 243(3):261-9

 [10] Taura T., Yoshihisa T., Ito K., 1997, Protein translocation functions of Escherichia coli SecY: in vitro characterization of cold-sensitive secY mutants., Biochimie 79(8):517-21

 [11] Veenendaal AK., van der Does C., Driessen AJ., 2001, Mapping the sites of interaction between SecY and SecE by cysteine scanning mutagenesis., J Biol Chem 276(35):32559-66

 [12] Watanabe M., Blobel G., 1989, Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins., Proc Natl Acad Sci U S A 86(6):1895-9

 [13] van der Sluis EO., Nouwen N., Driessen AJ., 2002, SecY-SecY and SecY-SecG contacts revealed by site-specific crosslinking., FEBS Lett 527(1-3):159-65

 [14] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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