RegulonDB RegulonDB 11.1: Gene Form
   

pspA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pspF pspA pspB IHF PspF PspF pspFp3 pspFp3 pspAp pspAp pspFp1 pspFp1 pspFp2 pspFp2

Gene      
Name: pspA    Texpresso search in the literature
Synonym(s): ECK1299, EG10776, b1304, cog
Genome position(nucleotides): 1368079 --> 1368747
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.33
External database links:  
ASAP:
 ABE-0004387
CGSC:
 32009
ECHOBASE:
 EB0769
ECOLIHUB:
 pspA
OU-MICROARRAY:
 b1304
STRING:
 511145.b1304
COLOMBOS: pspA


Product      
Name: phage shock protein A
Synonym(s): Cog, PspA, regulatory protein for the phage shock operon, protects membrane integrity
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 25.493
Isoelectric point: 5.156
Motif(s):
 
Type Positions Sequence Comment
2 -> 216 GIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSTSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIKSLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGKLDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAK
182 -> 182 A UniProt: In Ref. 1; CAA40789..

 
Classification:
Multifun Terms (GenProtEC)  
  3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.3 - inhibition / activation of enzymes
  3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
Gene Ontology Terms (GO)  
cellular_component GO:0005667 - transcription regulator complex
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0031234 - extrinsic component of cytoplasmic side of plasma membrane
GO:0060187 - cell pole
molecular_function GO:0005515 - protein binding
GO:0042802 - identical protein binding
GO:0005543 - phospholipid binding
biological_process GO:0006355 - regulation of transcription, DNA-templated
GO:0009408 - response to heat
GO:0043433 - negative regulation of DNA-binding transcription factor activity
GO:0080135 - regulation of cellular response to stress
GO:0009271 - phage shock
Note(s): Note(s): ...[more].
Evidence: [EXP-IDA] Inferred from direct assay
Reference(s): [1] Aoki SK., et al., 2009
[2] Dhamdhere G., et al., 2010
[3] Elderkin S., et al., 2002
[4] Engl C., et al., 2011
[5] Huvet M., et al., 2009
[6] Jovanovic G., et al., 2010
[7] Kim JS., et al., 2015
[8] Lenn T., et al., 2011
External database links:  
ALPHAFOLD:
 P0AFM6
DIP:
 DIP-10587N
ECOCYC:
 EG10776-MONOMER
ECOLIWIKI:
 b1304
INTERPRO:
 IPR007157
INTERPRO:
 IPR014319
PANTHER:
 PTHR31088
PDB:
 4WHE
PFAM:
 PF04012
PRIDE:
 P0AFM6
PRODB:
 PRO_000023614
REFSEQ:
 NP_415820
SMR:
 P0AFM6
UNIPROT:
 P0AFM6


Operon      
Name: pspABCDE         
Operon arrangement:
Transcription unit        Promoter
pspABCDE
pspBCDE
pspE


Transcriptional Regulation      
Display Regulation             
Activated by: IHF, PspF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Aoki SK., Webb JS., Braaten BA., Low DA., 2009, Contact-dependent growth inhibition causes reversible metabolic downregulation in Escherichia coli., J Bacteriol 191(6):1777-86

 [2] Dhamdhere G., Zgurskaya HI., 2010, Metabolic shutdown in Escherichia coli cells lacking the outer membrane channel TolC., Mol Microbiol 77(3):743-54

 [3] Elderkin S., Jones S., Schumacher J., Studholme D., Buck M., 2002, Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF., J Mol Biol 320(1):23-37

 [4] Engl C., Beek AT., Bekker M., de Mattos JT., Jovanovic G., Buck M., 2011, Dissipation of proton motive force is not sufficient to induce the phage shock protein response in Escherichia coli., Curr Microbiol 62(5):1374-85

 [5] Huvet M., Toni T., Tan H., Jovanovic G., Engl C., Buck M., Stumpf MP., 2009, Model-based evolutionary analysis: the natural history of phage-shock stress response., Biochem Soc Trans 37(Pt 4):762-7

 [6] Jovanovic G., Engl C., Mayhew AJ., Burrows PC., Buck M., 2010, Properties of the phage-shock-protein (Psp) regulatory complex that govern signal transduction and induction of the Psp response in Escherichia coli., Microbiology 156(Pt 10):2920-32

 [7] Kim JS., Jeong MK., Koo BS., Lee HC., 2015, Development of a Novel Plasmid-Free Thymidine Producer by Reprogramming Nucleotide Metabolic Pathways., Appl Environ Microbiol 81(22):7708-19

 [8] Lenn T., Gkekas CN., Bernard L., Engl C., Jovanovic G., Buck M., Ying L., 2011, Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching., Chem Commun (Camb) 47(1):400-2

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