RegulonDB RegulonDB 11.1: Gene Form
   

pstA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pstS pstC pstA pstB terminator pstCp2 pstCp2 pstCp pstCp pstBp2 pstBp2 pstBp1 pstBp1 pstBp3 pstBp3 phoUp phoUp phoUp2 phoUp2

Gene      
Name: pstA    Texpresso search in the literature
Synonym(s): ECK3719, EG10782, R2pho, b3726, phoR2, phoR2b, phoT
Genome position(nucleotides): 3908549 <-- 3909439
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.23
External database links:  
ASAP:
 ABE-0012184
CGSC:
 18007
ECHOBASE:
 EB0775
ECOLIHUB:
 pstA
OU-MICROARRAY:
 b3726
STRING:
 511145.b3726
COLOMBOS: pstA


Product      
Name: phosphate ABC transporter membrane subunit PstA
Synonym(s): PhoT, PstA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 32.322
Isoelectric point: 10.496
Motif(s):
 
Type Positions Sequence Comment
29 -> 50 IALTLSMATMAFGLFWLIWILM UniProt: Helical.
83 -> 102 LAGSGLLILWATVFGTPLGI UniProt: Helical.
101 -> 289 GIMAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIALALLQVPIVIRTTENMLKLVPYSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAIARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIITLCVLLLNILARVV
127 -> 146 ILLSAPSIVVGLFVYTIVVA UniProt: Helical.
151 -> 169 FSGWAGVIALALLQVPIVI UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.1 - phosphorous metabolism
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.m - ABC superfamily, membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
molecular_function GO:0015415 - ATPase-coupled phosphate ion transmembrane transporter activity
GO:0005315 - inorganic phosphate transmembrane transporter activity
biological_process GO:0010921 - regulation of phosphatase activity
GO:0006974 - cellular response to DNA damage stimulus
GO:0006817 - phosphate ion transport
GO:0055085 - transmembrane transport
GO:0035435 - phosphate ion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Webb DC., et al., 1992
External database links:  
ALPHAFOLD:
 P07654
ECOCYC:
 PSTA-MONOMER
ECOLIWIKI:
 b3726
INTERPRO:
 IPR000515
INTERPRO:
 IPR005672
INTERPRO:
 IPR035906
PFAM:
 PF00528
PRIDE:
 P07654
PRODB:
 PRO_000023621
PROSITE:
 PS50928
REFSEQ:
 NP_418182
UNIPROT:
 P07654


Operon      
Name: pstSCAB-phoU         
Operon arrangement:
Transcription unit        Promoter
phoU
pstB-phoU
pstB-phoU
pstB-phoU
pstCAB-phoU
pstCAB-phoU
pstS
pstSCA
pstSCAB-phoU
pstS
pstSCA
pstSCAB-phoU


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, IHF, PhoB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter phoUp2 3907646 reverse nd [COMP-AINF] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Webb DC., Rosenberg H., Cox GB., 1992, Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices., J Biol Chem 267(34):24661-8

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB