RegulonDB

Gene
Name:	recO
Synonym(s):	ECK2563, EG10832, b2565
Genome position(nucleotides):	2701741 <-- 2702469 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.05
External database links:

ASAP:	ABE-0008438
CGSC:	17962
ECHOBASE:	EB0825
ECOLIHUB:	recO
OU-MICROARRAY:	b2565
STRING:	511145.b2565
COLOMBOS:	recO

Product

Name:

DNA repair protein RecO

Synonym(s):

RecO

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

bacterial nucleoid,cytosol

Molecular weight:

27.391

Isoelectric point:

10.233

Motif(s):

Type	Positions	Sequence
	83 -> 226	GITLYSGLYINELLSRVLEYETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDDTMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLGGKPLKSRE
	4 -> 76	WQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRSTLKGALQPFTPLLLRFGGRGEVKTLRSAEAVS

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.1 - DNA related --> 2.1.3 - DNA recombination
		2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0043590 - bacterial nucleoid
	molecular_function	GO:0005515 - protein binding
	biological_process	GO:0006281 - DNA repair GO:0006310 - DNA recombination GO:0006974 - cellular response to DNA damage stimulus GO:0009314 - response to radiation GO:0006302 - double-strand break repair

Reference(s):

[1] Bork JM., et al., 2001
[2] Hegde S., et al., 1995
[3] Kolodner R., et al., 1985
[4] Luisi-DeLuca C. 1995
[5] Luisi-DeLuca C., et al., 1994
[6] Shan Q., et al., 1997

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PFAM:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

rnc-era-recO-pdxJ-acpS

Operon arrangement:

Transcription unit	Promoter
pdxJ-acpS	pdxJp
rnc-era	rncp
rnc-era-recO-pdxJ-acpS	rncp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	acpSp2	2701137	reverse	nd	[ICWHO]	[7]
promoter	acpSp3	2701162	reverse	nd	[ICWHO]	[7]
promoter	TSS_2856	2702292	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_2857	2702302	reverse	nd	[RS-EPT-CBR]	[8]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Bork JM., Cox MM., Inman RB., 2001, The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA., EMBO J 20(24):7313-22
[2] Hegde S., Sandler SJ., Clark AJ., Madiraju MV., 1995, recO and recR mutations delay induction of the SOS response in Escherichia coli., Mol Gen Genet 246(2):254-8
[3] Kolodner R., Fishel RA., Howard M., 1985, Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli., J Bacteriol 163(3):1060-6
[4] Luisi-DeLuca C., 1995, Homologous pairing of single-stranded DNA and superhelical double-stranded DNA catalyzed by RecO protein from Escherichia coli., J Bacteriol 177(3):566-72
[5] Luisi-DeLuca C., Kolodner R., 1994, Purification and characterization of the Escherichia coli RecO protein. Renaturation of complementary single-stranded DNA molecules catalyzed by the RecO protein., J Mol Biol 236(1):124-38
[6] Shan Q., Bork JM., Webb BL., Inman RB., Cox MM., 1997, RecA protein filaments: end-dependent dissociation from ssDNA and stabilization by RecO and RecR proteins., J Mol Biol 265(5):519-40
[7] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.