RegulonDB RegulonDB 11.1: Gene Form
   

rplB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rplB rplW rpsS rplD TSS_3811 (cluster) TSS_3811 (cluster) TSS_3810 TSS_3810 TSS_3809 (cluster) TSS_3809 (cluster) TSS_3808 TSS_3808 TSS_3807 (cluster) TSS_3807 (cluster) TSS_3806 TSS_3806 TSS_3805 TSS_3805 TSS_3804 TSS_3804 TSS_3803 TSS_3803 TSS_3802 TSS_3802 TSS_3801 TSS_3801 TSS_3800 (cluster) TSS_3800 (cluster)

Gene      
Name: rplB    Texpresso search in the literature
Synonym(s): ECK3304, EG10865, b3317
Genome position(nucleotides): 3450543 <-- 3451364
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.68
External database links:  
ASAP:
 ABE-0010854
CGSC:
 262
ECHOBASE:
 EB0858
ECOLIHUB:
 rplB
OU-MICROARRAY:
 b3317
STRING:
 511145.b3317
COLOMBOS: rplB


Product      
Name: 50S ribosomal subunit protein L2
Synonym(s): RplB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,ribosome
Molecular weight: 29.86
Isoelectric point: 11.55
Motif(s):
 
Type Positions Sequence Comment
2 -> 273 AVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK UniProt: 50S ribosomal protein L2.
28 -> 53 KPFAPLLEKNSKSGGRNNNGRITTRH UniProt: Disordered; Sequence Annotation Type: region of interest.
42 -> 117 GRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQ
125 -> 249 KPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWG
221 -> 273 RGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK UniProt: Disordered; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
  2 - information transfer --> 2.3 - protein related --> 2.3.8 - ribosomal proteins
  6 - cell structure --> 6.6 - ribosomes
Gene Ontology Terms (GO)  
cellular_component GO:1990082 - DnaA-L2 complex
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005840 - ribosome
GO:0015934 - large ribosomal subunit
GO:0022625 - cytosolic large ribosomal subunit
molecular_function GO:0003735 - structural constituent of ribosome
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0003723 - RNA binding
GO:0019843 - rRNA binding
GO:0008270 - zinc ion binding
biological_process GO:0006412 - translation
GO:0000027 - ribosomal large subunit assembly
GO:0032297 - negative regulation of DNA-templated DNA replication initiation
GO:0002181 - cytoplasmic translation
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P60422
DIP:
 DIP-35747N
ECOCYC:
 EG10865-MONOMER
ECOLIWIKI:
 b3317
INTERPRO:
 IPR005880
INTERPRO:
 IPR022669
INTERPRO:
 IPR002171
INTERPRO:
 IPR022671
INTERPRO:
 IPR008991
INTERPRO:
 IPR012340
INTERPRO:
 IPR014722
INTERPRO:
 IPR014726
INTERPRO:
 IPR022666
MODBASE:
 P60422
PANTHER:
 PTHR13691
PDB:
 2J28
PDB:
 2RDO
PDB:
 3BBX
PDB:
 3J5L
PDB:
 3J7Z
PDB:
 3J8G
PDB:
 3J9Y
PDB:
 3J9Z
PDB:
 3JA1
PDB:
 3JBU
PDB:
 3JBV
PDB:
 3JCD
PDB:
 3JCE
PDB:
 3JCJ
PDB:
 3JCN
PDB:
 487D
PDB:
 4CSU
PDB:
 4U1U
PDB:
 4U1V
PDB:
 4U20
PDB:
 4U24
PDB:
 4U25
PDB:
 4U26
PDB:
 4U27
PDB:
 4UY8
PDB:
 4V47
PDB:
 4V48
PDB:
 4V4H
PDB:
 4V4Q
PDB:
 4V4V
PDB:
 4V4W
PDB:
 4V50
PDB:
 4V52
PDB:
 4V53
PDB:
 4V54
PDB:
 4V55
PDB:
 4V56
PDB:
 4V57
PDB:
 4V5B
PDB:
 4V5H
PDB:
 4V5Y
PDB:
 4V64
PDB:
 4V65
PDB:
 4V66
PDB:
 4V69
PDB:
 4V6C
PDB:
 4V6D
PDB:
 4V6E
PDB:
 4V6K
PDB:
 4V6L
PDB:
 4V6M
PDB:
 4V6N
PDB:
 4V6O
PDB:
 4V6P
PDB:
 4V6Q
PDB:
 4V6R
PDB:
 4V6S
PDB:
 4V6T
PDB:
 4V6V
PDB:
 4V6Y
PDB:
 4V6Z
PDB:
 4V70
PDB:
 4V71
PDB:
 4V72
PDB:
 4V73
PDB:
 4V74
PDB:
 4V75
PDB:
 4V76
PDB:
 4V77
PDB:
 4V78
PDB:
 4V79
PDB:
 4V7A
PDB:
 4V7B
PDB:
 4V7C
PDB:
 4V7D
PDB:
 4V7I
PDB:
 4V7S
PDB:
 4V7T
PDB:
 4V7U
PDB:
 4V7V
PDB:
 4V85
PDB:
 4V89
PDB:
 4V9C
PDB:
 4V9D
PDB:
 4V9O
PDB:
 4V9P
PDB:
 4WF1
PDB:
 4WOI
PDB:
 4WWW
PDB:
 4YBB
PDB:
 5ADY
PDB:
 5AFI
PDB:
 5AKA
PDB:
 5GAD
PDB:
 5GAE
PDB:
 5GAF
PDB:
 5GAG
PDB:
 5GAH
PDB:
 5H5U
PDB:
 5IQR
PDB:
 5IT8
PDB:
 5J5B
PDB:
 5J7L
PDB:
 5J88
PDB:
 5J8A
PDB:
 5J91
PDB:
 5JC9
PDB:
 5JTE
PDB:
 5JU8
PDB:
 5KCR
PDB:
 5KCS
PDB:
 5KPS
PDB:
 5KPV
PDB:
 5KPW
PDB:
 5KPX
PDB:
 5L3P
PDB:
 5LZA
PDB:
 5LZB
PDB:
 5LZC
PDB:
 5LZD
PDB:
 5LZE
PDB:
 5LZF
PDB:
 5MDV
PDB:
 5MDW
PDB:
 5MDY
PDB:
 5MDZ
PDB:
 5MGP
PDB:
 5NCO
PDB:
 5NP6
PDB:
 5NWY
PDB:
 5O2R
PDB:
 5U4I
PDB:
 5U9F
PDB:
 5U9G
PDB:
 5UYK
PDB:
 5UYL
PDB:
 5UYM
PDB:
 5UYN
PDB:
 5UYP
PDB:
 5UYQ
PDB:
 5WDT
PDB:
 5WE4
PDB:
 5WE6
PDB:
 5WFK
PDB:
 6BU8
PDB:
 6BY1
PDB:
 6C4H
PDB:
 6ENF
PDB:
 6ENJ
PDB:
 6ENU
PDB:
 6GBZ
PDB:
 6GC0
PDB:
 6GC4
PDB:
 6GC6
PDB:
 6GC8
PDB:
 6GWT
PDB:
 6GXM
PDB:
 6GXN
PDB:
 6GXO
PDB:
 6GXP
PDB:
 6H4N
PDB:
 6H58
PDB:
 6HRM
PDB:
 6I0Y
PDB:
 6I7V
PDB:
 6O9J
PDB:
 6O9K
PDB:
 6OFX
PDB:
 6OG7
PDB:
 6OGF
PDB:
 6OGG
PDB:
 6OGI
PDB:
 6OM6
PDB:
 6ORE
PDB:
 6ORL
PDB:
 6OST
PDB:
 6OT3
PDB:
 6OUO
PDB:
 6PC5
PDB:
 6PC6
PDB:
 6PC7
PDB:
 6PC8
PDB:
 6PCH
PDB:
 6PCQ
PDB:
 6PCR
PDB:
 6PCS
PDB:
 6PCT
PDB:
 6Q98
PDB:
 6Q9A
PDB:
 6QDW
PDB:
 6QUL
PDB:
 6S0K
PDB:
 6SZS
PDB:
 6TBV
PDB:
 6TC3
PDB:
 6VU3
PDB:
 6VWL
PDB:
 6VWM
PDB:
 6VWN
PDB:
 6VYQ
PDB:
 6VYR
PDB:
 6VYS
PDB:
 6VYT
PDB:
 6VYU
PDB:
 6VYW
PDB:
 6VYX
PDB:
 6VYY
PDB:
 6VYZ
PDB:
 6VZ2
PDB:
 6VZ3
PDB:
 6VZ5
PDB:
 6VZ7
PDB:
 6VZJ
PDB:
 6WD0
PDB:
 6WD1
PDB:
 6WD2
PDB:
 6WD3
PDB:
 6WD4
PDB:
 6WD5
PDB:
 6WD6
PDB:
 6WD7
PDB:
 6WD8
PDB:
 6WD9
PDB:
 6WDA
PDB:
 6WDB
PDB:
 6WDC
PDB:
 6WDD
PDB:
 6WDE
PDB:
 6WDF
PDB:
 6WDG
PDB:
 6WDH
PDB:
 6WDI
PDB:
 6WDJ
PDB:
 6WDK
PDB:
 6WDL
PDB:
 6WDM
PDB:
 6WNT
PDB:
 6WNV
PDB:
 6WNW
PDB:
 6WYV
PDB:
 6X6T
PDB:
 6X7F
PDB:
 6X7K
PDB:
 6X9Q
PDB:
 6XDQ
PDB:
 6XDR
PDB:
 6XGF
PDB:
 6XII
PDB:
 6XIJ
PDB:
 6XZ7
PDB:
 6XZA
PDB:
 6XZB
PDB:
 6Y69
PDB:
 6YS3
PDB:
 6YSR
PDB:
 6YSS
PDB:
 6YST
PDB:
 6YSU
PDB:
 6ZTJ
PDB:
 6ZTL
PDB:
 6ZTM
PDB:
 6ZTN
PDB:
 6ZTO
PDB:
 6ZTP
PDB:
 6ZU1
PDB:
 7BV8
PDB:
 7JSS
PDB:
 7JSW
PDB:
 7JSZ
PDB:
 7JT1
PDB:
 7JT2
PDB:
 7JT3
PDB:
 7K00
PFAM:
 PF00181
PFAM:
 PF03947
PRIDE:
 P60422
PRODB:
 PRO_000023810
PROSITE:
 PS00467
REFSEQ:
 NP_417776
SMART:
 SM01382
SMART:
 SM01383
SMR:
 P60422
UNIPROT:
 P60422


Operon      
Name: rpsJ-rplCDWB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ         
Operon arrangement:
Transcription unit        Promoter
rpsJ-rplCDWB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ


Transcriptional Regulation      
Display Regulation             
Activated by: FNR
Repressed by: ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3800 (cluster) 3450252 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3801 3450347 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3802 3450357 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3803 3450389 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3804 3450425 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3805 3450474 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3806 3450533 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3807 (cluster) 3450543 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3808 3451410 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3809 (cluster) 3451437 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3810 3451529 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3811 (cluster) 3451535 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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