RegulonDB RegulonDB 10.9: Gene Form
   

secE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tufB nusG secE DksA-ppGpp DksA-ppGpp anti-anti-terminator terminator anti-terminator anti-anti-terminator TSS_4736 TSS_4736 TSS_4735 TSS_4735 TSS_4734 (cluster) TSS_4734 (cluster) TSS_4733 (cluster) TSS_4733 (cluster) TSS_4732 (cluster) TSS_4732 (cluster) TSS_4731 (cluster) TSS_4731 (cluster) TSS_4730 TSS_4730 TSS_4729 (cluster) TSS_4729 (cluster) TSS_4728 TSS_4728 secEp secEp TSS_4727 TSS_4727 TSS_4726 TSS_4726 TSS_4725 TSS_4725 TSS_4724 TSS_4724 TSS_4723 (cluster) TSS_4723 (cluster) TSS_4722 (cluster) TSS_4722 (cluster) TSS_4721 TSS_4721 TSS_4720 (cluster) TSS_4720 (cluster) TSS_4719 TSS_4719 TSS_4718 (cluster) TSS_4718 (cluster) TSS_4717 TSS_4717 TSS_4716 TSS_4716 TSS_4715 TSS_4715 TSS_4714 TSS_4714 TSS_4713 TSS_4713 TSS_4712 TSS_4712 TSS_4711 TSS_4711 TSS_4710 TSS_4710 TSS_4709 (cluster) TSS_4709 (cluster)

Gene      
Name: secE    Texpresso search in the literature
Synonym(s): ECK3972, EG10939, b3981, mbrC, prlG
Genome position(nucleotides): 4177358 --> 4177741 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.95
External database links:  
ASAP:
ABE-0013023
CGSC:
34108
ECHOBASE:
EB0932
ECOLIHUB:
secE
OU-MICROARRAY:
b3981
STRING:
511145.b3981
COLOMBOS: secE


Product      
Name: Sec translocon subunit SecE
Synonym(s): MbrC, PrlG, SecE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 13.643
Isoelectric point: 11.155
Motif(s):
 
Type Positions Sequence
78 -> 78 E
98 -> 115 AAVTAVMSLILWGLDGIL
85 -> 85 P
105 -> 105 S
120 -> 120 S

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.5 - The Type II (General) Secretory Pathway (IISP) Family
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0031522 - cell envelope Sec protein transport complex
molecular_function GO:0005515 - protein binding
GO:0008320 - protein transmembrane transporter activity
GO:0015450 - P-P-bond-hydrolysis-driven protein transmembrane transporter activity
biological_process GO:0015031 - protein transport
GO:0006605 - protein targeting
GO:0006886 - intracellular protein transport
GO:0009306 - protein secretion
GO:0065002 - intracellular protein transmembrane transport
GO:0032978 - protein insertion into membrane from inner side
GO:0043952 - protein transport by the Sec complex
GO:0006616 - SRP-dependent cotranslational protein targeting to membrane, translocation
Note(s): Note(s): ...[more].
Reference(s): [1] Baars L., et al., 2008
[2] Bieker KL., et al., 1990
[3] Flower AM., et al., 1994
[4] Flower AM., et al., 1995
[5] Kaufmann A., et al., 1999
[6] Kontinen VP., et al., 1996
[7] Matsuyama S., et al., 1990
[8] Murphy CK., et al., 1994
[9] Nishiyama K., et al., 1995
[10] Pohlschroder M., et al., 1996
[11] Veenendaal AK., et al., 2002
External database links:  
DIP:
DIP-59303N
ECOCYC:
SECE
ECOLIWIKI:
b3981
INTERPRO:
IPR005807
INTERPRO:
IPR001901
INTERPRO:
IPR038379
PANTHER:
PTHR33910
PDB:
6R7L
PDB:
5NCO
PDB:
5MG3
PDB:
5GAE
PDB:
4V6M
PDB:
3J46
PDB:
3J45
PDB:
2AKI
PDB:
2AKH
PFAM:
PF00584
PRIDE:
P0AG96
PRINTS:
PR01650
PRODB:
PRO_000023926
PROSITE:
PS01067
REFSEQ:
NP_418408
SMR:
P0AG96
UNIPROT:
P0AG96


Operon      
Name: secE-nusG         
Operon arrangement:
Transcription unit        Promoter
secE-nusG


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4709 (cluster) 4175958 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4710 4175968 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4711 4176116 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4712 4176325 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4713 4176332 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4714 4176429 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4715 4176443 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4716 4176445 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4717 4176452 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4718 (cluster) 4176586 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4719 4176591 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4720 (cluster) 4176593 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4721 4176597 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4722 (cluster) 4176659 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4723 (cluster) 4176669 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4724 4176704 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4725 4176727 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4726 4176739 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4727 4176975 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4728 4177213 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4729 (cluster) 4177243 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4730 4177246 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4731 (cluster) 4177278 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4732 (cluster) 4177295 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4733 (cluster) 4177301 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4734 (cluster) 4177329 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter TSS_4735 4177340 forward nd [RS-EPT-CBR] [12]
  promoter TSS_4736 4177443 reverse nd [RS-EPT-CBR] [12]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Baars L., Wagner S., Wickstrom D., Klepsch M., Ytterberg AJ., van Wijk KJ., de Gier JW., 2008, Effects of SecE depletion on the inner and outer membrane proteomes of Escherichia coli., J Bacteriol 190(10):3505-25

 [2] Bieker KL., Silhavy TJ., 1990, PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli., Cell 61(5):833-42

 [3] Flower AM., Doebele RC., Silhavy TJ., 1994, PrlA and PrlG suppressors reduce the requirement for signal sequence recognition., J Bacteriol 176(18):5607-14

 [4] Flower AM., Osborne RS., Silhavy TJ., 1995, The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE., EMBO J 14(5):884-93

 [5] Kaufmann A., Manting EH., Veenendaal AK., Driessen AJ., van der Does C., 1999, Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE., Biochemistry 38(28):9115-25

 [6] Kontinen VP., Yamanaka M., Nishiyama K., Tokuda H., 1996, Roles of the conserved cytoplasmic region and non-conserved carboxy-terminal region of SecE in Escherichia coli protein translocase., J Biochem 119(6):1124-30

 [7] Matsuyama S., Akimaru J., Mizushima S., 1990, SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machinery., FEBS Lett 269(1):96-100

 [8] Murphy CK., Beckwith J., 1994, Residues essential for the function of SecE, a membrane component of the Escherichia coli secretion apparatus, are located in a conserved cytoplasmic region., Proc Natl Acad Sci U S A 91(7):2557-61

 [9] Nishiyama K., Mizushima S., Tokuda H., 1995, Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane., Biochem Biophys Res Commun 217(1):217-23

 [10] Pohlschroder M., Murphy C., Beckwith J., 1996, In vivo analyses of interactions between SecE and SecY, core components of the Escherichia coli protein translocation machinery., J Biol Chem 271(33):19908-14

 [11] Veenendaal AK., Van Der Does C., Driessen AJ., 2002, The core of the bacterial translocase harbors a tilted transmembrane segment 3 of SecE., J Biol Chem 277(39):36640-5

 [12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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