RegulonDB

Gene
Name:	gutM
Synonym(s):	ECK2701, EG10972, b2706, gut, srlM
Genome position(nucleotides):	2828621 --> 2828980 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.0
External database links:

ASAP:	ABE-0008898
CGSC:	33198
ECHOBASE:	EB0965
ECOLIHUB:	gutM
OU-MICROARRAY:	b2706
STRING:	511145.b2706
COLOMBOS:	gutM

Product

Name:

DNA-binding transcriptional activator GutM

Synonym(s):

Gut, GutM, SrlM

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

GutM

Molecular weight:

12.953

Isoelectric point:

11.199

Motif(s):

Type	Positions	Sequence
	5 -> 110	LITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGRSSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPHDPLSQNAL
	23 -> 29	QISRFNR

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
Gene Ontology Terms (GO)
	molecular_function	GO:0003677 - DNA binding
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system

Note(s):

Note(s): ...[more].

Evidence:

[HIFS] Human inference of function from sequence
[IEP] Inferred from expression pattern

Reference(s):

[1] Yamada M., et al., 1988

External database links:

ECOCYC:

ECOLIWIKI:

ECOO157CYC:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

srlAEBD-gutM-srlR-gutQ

Operon arrangement:

Transcription unit	Promoter
srlAEBD-gutM-srlR-gutQ	srlAp
srlR	srlRp

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, H-NS, GutM
Repressed by:	SrlR

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_3017 (cluster)	2828961	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2828961 to 2828962, where the highest frequency is at position 2826983. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
promoter	TSS_3018 (cluster)	2829709	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2829709 to 2829710, where the highest frequency is at position 2827732. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
promoter	TSS_3019	2829746	forward	nd	[RS-EPT-CBR]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Yamada M., Saier MH., 1988, Positive and negative regulators for glucitol (gut) operon expression in Escherichia coli., J Mol Biol 203(3):569-83
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Yamada M., Saier MH., 1988, Positive and negative regulators for glucitol (gut) operon expression in Escherichia coli., J Mol Biol 203(3):569-83

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.