RegulonDB RegulonDB 11.1: Gene Form
   

tgt gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tgt queA yajC terminator anti-terminator anti-anti-terminator yajCp yajCp TSS_511 TSS_511 TSS_510 TSS_510 tgtp tgtp

Gene      
Name: tgt    Texpresso search in the literature
Synonym(s): ECK0400, EG10996, b0406, vacC(S.f.)
Genome position(nucleotides): 426137 --> 427264
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.33
External database links:  
ASAP:
 ABE-0001413
CGSC:
 118
ECHOBASE:
 EB0989
ECOLIHUB:
 tgt
OU-MICROARRAY:
 b0406
STRING:
 511145.b0406
COLOMBOS: tgt


Product      
Name: tRNA-guanine transglycosylase
Synonym(s): Tgt
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 42.594
Isoelectric point: 6.351
Motif(s):
 
Type Positions Sequence Comment
11 -> 364 RARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQR
28 -> 28 C UniProt: Slight loss of activity..
54 -> 55 IL UniProt: In Ref. 1 and 2..
89 -> 89 D UniProt: Reduces catalytic activity by 51%..
90 -> 90 S UniProt: Loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016757 - glycosyltransferase activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016763 - pentosyltransferase activity
GO:0008270 - zinc ion binding
GO:0008479 - queuine tRNA-ribosyltransferase activity
biological_process GO:0101030 - tRNA-guanine transglycosylation
GO:0006400 - tRNA modification
GO:0008033 - tRNA processing
GO:0008616 - queuosine biosynthetic process
GO:0002099 - tRNA wobble guanine modification
Reference(s): [1] Chong S., et al., 1994
[2] Dineshkumar TK., et al., 2002
[3] Janel G., et al., 1984
[4] Urbonavicius J., et al., 2003
External database links:  
ALPHAFOLD:
 P0A847
DIP:
 DIP-36020N
ECOCYC:
 EG10996-MONOMER
INTERPRO:
 IPR002616
INTERPRO:
 IPR004803
INTERPRO:
 IPR036511
PFAM:
 PF01702
PRIDE:
 P0A847
REFSEQ:
 NP_414940
SMR:
 P0A847
UNIPROT:
 P0A847


Operon      
Name: tgt-yajC-secDF         
Operon arrangement:
Transcription unit        Promoter
tgt-yajC
yajC-secDF


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_510 426062 forward nd [RS-EPT-CBR] [5]
  promoter TSS_511 427204 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Chong S., Garcia GA., 1994, A versatile and general prokaryotic expression vector, pLACT7., Biotechniques 17(4):686, 688, 690-1

 [2] Dineshkumar TK., Thanedar S., Subbulakshmi C., Varshney U., 2002, An unexpected absence of queuosine modification in the tRNAs of an Escherichia coli B strain., Microbiology 148(Pt 12):3779-87

 [3] Janel G., Michelsen U., Nishimura S., Kersten H., 1984, Queuosine modification in tRNA and expression of the nitrate reductase in Escherichia coli., EMBO J 3(7):1603-8

 [4] Urbonavicius J., Stahl G., Durand JM., Ben Salem SN., Qian Q., Farabaugh PJ., Bjork GR., 2003, Transfer RNA modifications that alter +1 frameshifting in general fail to affect -1 frameshifting., RNA 9(6):760-8

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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