RegulonDB RegulonDB 10.9: Gene Form
   

mnmE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yidC mnmE tnaC TorR CRP TorR terminator anti-terminator anti-anti-terminator terminator tnaCp tnaCp TSS_4277 (cluster) TSS_4277 (cluster)

Gene      
Name: mnmE    Texpresso search in the literature
Synonym(s): ECK3699, EG10997, b3706, thdF, trmE
Genome position(nucleotides): 3886828 --> 3888192 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
56.34
External database links:  
ASAP:
ABE-0012123
CGSC:
17806
ECHOBASE:
EB0990
ECOLIHUB:
mnmE
OU-MICROARRAY:
b3706
STRING:
511145.b3706
COLOMBOS: mnmE


Product      
Name: 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit
Synonym(s): 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmE, GTPase, involved in modification of U34 in tRNA, MnmE, ThdF, TrmE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 49.231
Isoelectric point: 4.647
Motif(s):
 
Type Positions Sequence
216 -> 377 GMKVVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMG
228 -> 228 G
255 -> 255 L
270 -> 273 DTAG
358 -> 360 SAR

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
  2 - information transfer --> 2.2 - RNA related --> 2.2.5 - tRNA
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane
molecular_function GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0000166 - nucleotide binding
GO:0003924 - GTPase activity
GO:0005525 - GTP binding
GO:0030955 - potassium ion binding
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
GO:0019003 - GDP binding
biological_process GO:0006400 - tRNA modification
GO:0030488 - tRNA methylation
GO:0009268 - response to pH
GO:0008033 - tRNA processing
GO:0002098 - tRNA wobble uridine modification
GO:0061077 - chaperone-mediated protein folding
Note(s): Note(s): ...[more].
Reference(s): [1] Ash MR., et al., 2012
[2] Deng Z., et al., 2013
[3] Gruian C., et al., 2014
[4] Gruian C., et al., 2012
[5] Krasich R., et al., 2015
[6] Lee C., et al., 2016
[7] Scrima A., et al., 2005
External database links:  
DIP:
DIP-11033N
ECOCYC:
EG10997-MONOMER
ECOLIWIKI:
b3706
INTERPRO:
IPR027368
INTERPRO:
IPR031168
INTERPRO:
IPR027417
INTERPRO:
IPR027266
INTERPRO:
IPR025867
INTERPRO:
IPR018948
INTERPRO:
IPR006073
INTERPRO:
IPR005225
INTERPRO:
IPR004520
MODBASE:
P25522
PDB:
2GJ8
PDB:
2GJ9
PDB:
2GJA
PDB:
1RFL
PFAM:
PF10396
PFAM:
PF12631
PFAM:
PF01926
PRIDE:
P25522
PRODB:
PRO_000023256
PROSITE:
PS51709
REFSEQ:
NP_418162
SMR:
P25522
UNIPROT:
P25522


Operon      
Name: mnmE         
Operon arrangement:
Transcription unit        Promoter
mnmE


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4277 (cluster) 3886426 forward For this promoter, there
Read more >
[RS-EPT-CBR] [8]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Ash MR., Maher MJ., Mitchell Guss J., Jormakka M., 2012, The cation-dependent G-proteins: In a class of their own., FEBS Lett 586(16):2218-24

 [2] Deng Z., Shan Y., Pan Q., Gao X., Yan A., 2013, Anaerobic expression of the gadE-mdtEF multidrug efflux operon is primarily regulated by the two-component system ArcBA through antagonizing the H-NS mediated repression., Front Microbiol 4:194

 [3] Gruian C., Boehme S., Simon S., Steinhoff HJ., Klare JP., 2014, Assembly and function of the tRNA-modifying GTPase MnmE adsorbed to surface functionalized bioactive glass., ACS Appl Mater Interfaces 6(10):7615-25

 [4] Gruian C., Vanea E., Simon S., Simon V., 2012, FTIR and XPS studies of protein adsorption onto functionalized bioactive glass., Biochim Biophys Acta 1824(7):873-81

 [5] Krasich R., Wu SY., Kuo HK., Kreuzer KN., 2015, Functions that protect Escherichia coli from DNA-protein crosslinks., DNA Repair (Amst) 28:48-59

 [6] Lee C., Kwon M., Lee K., Min H., Kim SH., Lee N., Kim J., Kim D., Ko C., Park C., 2016, Screening for Escherichia coli K-12 genes conferring glyoxal resistance or sensitivity by transposon insertions., FEMS Microbiol Lett 363(18)

 [7] Scrima A., Vetter IR., Armengod ME., Wittinghofer A., 2005, The structure of the TrmE GTP-binding protein and its implications for tRNA modification., EMBO J 24(1):23-33

 [8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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