RegulonDB RegulonDB 10.10: Gene Form
   

ispH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ispH rihC fkpB TSS_73 TSS_73

Gene      
Name: ispH    Texpresso search in the literature
Synonym(s): ECK0030, EG11081, b0029, lytB, yaaE
Genome position(nucleotides): 26277 --> 27227 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.1
External database links:  
ASAP:
 ABE-0000103
CGSC:
 34696
ECHOBASE:
 EB1073
ECOLIHUB:
 ispH
OU-MICROARRAY:
 b0029
STRING:
 511145.b0029
COLOMBOS: ispH


Product      
Name: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Synonym(s): 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, IspH, LytB, YaaE
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 34.775
Isoelectric point: 4.952
Motif(s):
 
Type Positions Sequence
12 -> 12 C
41 -> 41 H
3 -> 282 ILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRYVVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEKLSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVLVVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQNVVARL
96 -> 96 C
197 -> 197 C

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.11 - menaquinone, ubiquinone
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0042380 - hydroxymethylbutenyl pyrophosphate reductase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0051538 - 3 iron, 4 sulfur cluster binding
GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
biological_process GO:0008299 - isoprenoid biosynthetic process
GO:0016114 - terpenoid biosynthetic process
GO:0019288 - isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0050992 - dimethylallyl diphosphate biosynthetic process
External database links:  
DIP:
 DIP-35808N
ECOCYC:
 EG11081-MONOMER
ECOLIWIKI:
 b0029
INTERPRO:
 IPR003451
PANTHER:
 PTHR30426
PDB:
 4H4E
PDB:
 4EB3
PDB:
 4H4C
PDB:
 4H4D
PDB:
 3F7T
PDB:
 3KE8
PDB:
 3KE9
PDB:
 3KEF
PDB:
 3KEL
PDB:
 3KEM
PDB:
 3SZL
PDB:
 3SZO
PDB:
 3SZU
PDB:
 3T0F
PDB:
 3T0G
PDB:
 3URK
PDB:
 3UTC
PDB:
 3UTD
PDB:
 3UV3
PDB:
 3UV6
PDB:
 3UV7
PDB:
 3UWM
PDB:
 3ZGL
PDB:
 3ZGN
PFAM:
 PF02401
PRIDE:
 P62623
PRODB:
 PRO_000023040
REFSEQ:
 NP_414570
SMR:
 P62623
UNIPROT:
 P62623


Operon      
Name: ribF-ileS-lspA-fkpB-ispH         
Operon arrangement:
Transcription unit        Promoter
ribF-ileS-lspA-fkpB-ispH
ileS-lspA-fkpB-ispH
ileS-lspA-fkpB-ispH
ileS-lspA-fkpB-ispH
lsp-fkpB-ispH


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_73 27263 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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