RegulonDB RegulonDB 11.1: Gene Form
   

mukE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mukB ldtD mukF cmoM mukE CpxR ldtDp ldtDp TSS_1197 TSS_1197 TSS_1196 TSS_1196 TSS_1195 TSS_1195 TSS_1194 TSS_1194

Gene      
Name: mukE    Texpresso search in the literature
Synonym(s): ECK0914, EG11252, b0923, kicA, ycbA
Genome position(nucleotides): 975622 --> 976326
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.63
External database links:  
ASAP:
 ABE-0003136
CGSC:
 31751
ECHOBASE:
 EB1232
ECOLIHUB:
 mukE
OU-MICROARRAY:
 b0923
STRING:
 511145.b0923
COLOMBOS: mukE


Product      
Name: chromosome partitioning protein MukE
Synonym(s): KicA, MukE, YcbA, protein involved in chromosome partitioning
Sequence: Get amino acid sequence Fasta Format
Cellular location: bacterial nucleoid,cytosol
Molecular weight: 26.974
Isoelectric point: 4.635
Motif(s):
 
Type Positions Sequence Comment
10 -> 230 MPVKLAQALANPLFPALDSALRSGRHIGLDELDNHAFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYLYLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLNRLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQLNDETEENQPDS
207 -> 234 DGEAMPIENHLQLNDETEENQPDSGEEE UniProt: Disordered; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related
  3 - regulation --> 3.1 - type of regulation --> 3.1.4 - unknown
  5 - cell processes --> 5.1 - cell division
  5 - cell processes --> 5.10 - defense/survival
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0009295 - nucleoid
GO:0000796 - condensin complex
molecular_function GO:0005515 - protein binding
biological_process GO:0051301 - cell division
GO:0006260 - DNA replication
GO:0007049 - cell cycle
GO:0007059 - chromosome segregation
GO:0030261 - chromosome condensation
Note(s): Note(s): ...[more].
Reference(s): [1] Gloyd M., et al., 2011
[2] Svanberg Frisinger F., et al., 2021
External database links:  
ALPHAFOLD:
 P22524
DIP:
 DIP-10274N
ECOCYC:
 EG11252-MONOMER
ECOLIWIKI:
 b0923
INTERPRO:
 IPR042037
INTERPRO:
 IPR042038
INTERPRO:
 IPR007385
MINT:
 P22524
PDB:
 3RPU
PDB:
 3EUH
PFAM:
 PF04288
PRIDE:
 P22524
PRODB:
 PRO_000023310
REFSEQ:
 NP_415443
SMR:
 P22524
UNIPROT:
 P22524


Operon      
Name: cmoM-mukFEB         
Operon arrangement:
Transcription unit        Promoter
smtA-mukFEB


Transcriptional Regulation      
Display Regulation             
Activated by: H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1194 975868 forward nd [RS-EPT-CBR] [3]
  promoter TSS_1195 979917 forward nd [RS-EPT-CBR] [3]
  promoter TSS_1196 980088 forward nd [RS-EPT-CBR] [3]
  promoter TSS_1197 980108 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Gloyd M., Ghirlando R., Guarne A., 2011, The role of MukE in assembling a functional MukBEF complex., J Mol Biol 412(4):578-90

 [2] Svanberg Frisinger F., Jana B., Donadio S., Guardabassi L., 2021, In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli., Antibiotics (Basel) 10(6)

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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