Gene
Name:	nfsA
Synonym(s):	ECK0842, EG11261, b0851, mda18, mdaA, ybjB
Genome position(nucleotides):	891184 --> 891906
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.56
External database links:

ASAP:	ABE-0002900
ECHOBASE:	EB1241
ECOLIHUB:	nfsA
OU-MICROARRAY:	b0851
STRING:	511145.b0851
COLOMBOS:	nfsA

Transcriptional Regulation
	Display Regulation
Activated by:	MarA, SoxS, Rob
Repressed by:	OxyR

Reference(s)
[1] Chan-Hyams JVE., Ackerley DF., 2020, Protocol for evaluating the abilities of diverse nitroaromatic prodrug metabolites to exit a model Gram negative bacterial vector., MethodsX 7:100797
[2] Chan-Hyams JVE., Copp JN., Smaill JB., Patterson AV., Ackerley DF., 2018, Evaluating the abilities of diverse nitroaromatic prodrug metabolites to exit a model Gram negative vector for bacterial-directed enzyme-prodrug therapy., Biochem Pharmacol 158:192-200
[3] Copp JN., Mowday AM., Williams EM., Guise CP., Ashoorzadeh A., Sharrock AV., Flanagan JU., Smaill JB., Patterson AV., Ackerley DF., 2017, Engineering a Multifunctional Nitroreductase for Improved Activation of Prodrugs and PET Probes for Cancer Gene Therapy., Cell Chem Biol 24(3):391-403
[4] Copp JN., Williams EM., Rich MH., Patterson AV., Smaill JB., Ackerley DF., 2014, Toward a high-throughput screening platform for directed evolution of enzymes that activate genotoxic prodrugs., Protein Eng Des Sel 27(10):399-403
[5] Hameed P S., Bharatham N., Katagihallimath N., Sharma S., Nandishaiah R., Shanbhag AP., Thomas T., Narjari R., Sarma M., Bhowmik P., Amar P., Ravishankar R., Jayaraman R., Muthan K., Subbiah R., Ramachandran V., Balasubramanian V., Datta S., 2018, Nitrothiophene carboxamides, a novel narrow spectrum antibacterial series: Mechanism of action and Efficacy., Sci Rep 8(1):7263
[6] Hao Y., Updegrove TB., Livingston NN., Storz G., 2016, Protection against deleterious nitrogen compounds: role of σS-dependent small RNAs encoded adjacent to sdiA., Nucleic Acids Res 44(14):6935-48
[7] Hong CR., Dickson BD., Jaiswal JK., Pruijn FB., Hunter FW., Hay MP., Hicks KO., Wilson WR., 2018, Cellular pharmacology of evofosfamide (TH-302): A critical re-evaluation of its bystander effects., Biochem Pharmacol 156:265-280
[8] Li Z., Gao X., Shi W., Li X., Ma H., 2013, 7-((5-Nitrothiophen-2-yl)methoxy)-3H-phenoxazin-3-one as a spectroscopic off-on probe for highly sensitive and selective detection of nitroreductase., Chem Commun (Camb) 49(52):5859-61
[9] Merkley ED., Parson WW., Daggett V., 2010, Temperature dependence of the flexibility of thermophilic and mesophilic flavoenzymes of the nitroreductase fold., Protein Eng Des Sel 23(5):327-36
[10] Mowday AM., Ashoorzadeh A., Williams EM., Copp JN., Silva S., Bull MR., Abbattista MR., Anderson RF., Flanagan JU., Guise CP., Ackerley DF., Smaill JB., Patterson AV., 2016, Rational design of an AKR1C3-resistant analog of PR-104 for enzyme-prodrug therapy., Biochem Pharmacol 116:176-87
[11] Mowday AM., Copp JN., Syddall SP., Dubois LJ., Wang J., Lieuwes NG., Biemans R., Ashoorzadeh A., Abbattista MR., Williams EM., Guise CP., Lambin P., Ackerley DF., Smaill JB., Theys J., Patterson AV., 2020, E. coli nitroreductase NfsA is a reporter gene for non-invasive PET imaging in cancer gene therapy applications., Theranostics 10(23):10548-10562
[12] Pradhan SK., Singh NR., Dehury B., Panda D., Modi MK., Thatoi H., 2019, Insights into the mode of flavin mononucleotide binding and catalytic mechanism of bacterial chromate reductases: A molecular dynamics simulation study., J Cell Biochem 120(10):16990-17005
[13] Steenhuis M., Koningstein GM., Oswald J., Pick T., O'Keefe S., Koch HG., Cavalie A., Whitehead RC., Swanton E., High S., Luirink J., 2021, Eeyarestatin 24 impairs SecYEG-dependent protein trafficking and inhibits growth of clinically relevant pathogens., Mol Microbiol 115(1):28-40
[14] Valle A., Le Borgne S., Bolivar J., Cabrera G., Cantero D., 2012, Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties., Appl Microbiol Biotechnol 94(1):163-71