RegulonDB RegulonDB 11.1: Gene Form
   

tsgA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nirB tsgA ppiA nirD Fis Cra FNR CRP NarL NarP NarL NarP IHF Fis IHF Fis CRP CRP CytR CytR NsrR CRP CpxR RyhB terminator TSS_3976 TSS_3976 TSS_3975 TSS_3975 TSS_3974 TSS_3974 TSS_3973 TSS_3973 TSS_3972 TSS_3972 TSS_3971 TSS_3971 TSS_3970 TSS_3970 TSS_3969 TSS_3969 TSS_3968 (cluster) TSS_3968 (cluster) TSS_3967 TSS_3967 nirBp nirBp tsgAp tsgAp ppiAp4 ppiAp4 ppiAp3 ppiAp3 ppiAp2 ppiAp2 ppiAp1 ppiAp1

Gene      
Name: tsgA    Texpresso search in the literature
Synonym(s): ECK3352, EG11262, b3364, gutS, yhfC, yhfH
Genome position(nucleotides): 3492568 --> 3493749
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.86
External database links:  
ASAP:
 ABE-0010997
ECHOBASE:
 EB1242
ECOLIHUB:
 tsgA
OU-MICROARRAY:
 b3364
STRING:
 511145.b3364
COLOMBOS: tsgA


Product      
Name: putative transporter TsgA
Synonym(s): GutS, TsgA, YhfC, YhfH
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 43.166
Isoelectric point: 9.092
Motif(s):
 
Type Positions Sequence Comment
11 -> 31 WISFLSYALTGALVIVTGMVM UniProt: Helical.
14 -> 319 FLSYALTGALVIVTGMVMGNIADYFNLPVSSMSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAAMFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSIEWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCYILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTVLAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGS
51 -> 71 FLNAGILISIFLNAWLMEIVP UniProt: Helical.
78 -> 98 FGFLLMVLAVAGLMFSHSLAL UniProt: Helical.
101 -> 121 AAMFILGVVSGITMSIGTFLV UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0022857 - transmembrane transporter activity
biological_process GO:0055085 - transmembrane transport
GO:0072714 - response to selenite ion
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P60778
ECOCYC:
 YHFC-MONOMER
ECOLIWIKI:
 b3364
INTERPRO:
 IPR023528
INTERPRO:
 IPR020846
INTERPRO:
 IPR036259
INTERPRO:
 IPR011701
MODBASE:
 P60778
PFAM:
 PF07690
PRIDE:
 P60778
PRODB:
 PRO_000024133
PROSITE:
 PS50850
REFSEQ:
 NP_417823
UNIPROT:
 P60778


Operon      
Name: tsgA         
Operon arrangement:
Transcription unit        Promoter
tsgA


Transcriptional Regulation      
Display Regulation             
Repressed by: NsrR


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3967 3493998 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3968 (cluster) 3494032 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3969 3494035 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3970 3494404 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3971 3494422 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3972 3494535 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3973 3494981 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3974 3495073 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3975 3495419 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3976 3496522 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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