RegulonDB RegulonDB 10.9: Gene Form
   

pheM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pheS rplT pheM Cis-reg; leader anti-terminator terminator anti-anti-terminator anti-terminator terminator TSS_2013 TSS_2013 pheMp pheMp TSS_2012 TSS_2012 TSS_2011 TSS_2011 TSS_2010 TSS_2010

Gene      
Name: pheM    Texpresso search in the literature
Synonym(s): ECK1713, EG11272, b1715, phtL
Genome position(nucleotides): 1799226 <-- 1799270 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
35.56
External database links:  
ASAP:
ABE-0005727
ECHOBASE:
EB1251
ECOLIHUB:
pheM
OU-MICROARRAY:
b1715
COLOMBOS: pheM


Product      
Name: pheST-ihfA operon leader peptide
Synonym(s): PheM, PhtL, phenylalanyl-tRNA synthetase (pheST) operon leader peptide
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 1.762
Isoelectric point: 9.355
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.1 - amino acid -activation
Gene Ontology Terms (GO)  
biological_process GO:0006355 - regulation of transcription, DNA-templated
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Mayaux JF., et al., 1985
[2] Mayaux JF., et al., 1984
[3] Springer M., et al., 1987
[4] Springer M., et al., 1985
External database links:  
ECOCYC:
EG11272-MONOMER
ECOLIWIKI:
b1715
PRIDE:
P0AD74
REFSEQ:
NP_416230
UNIPROT:
P0AD74


Operon      
Name: thrS-infC-rpmI-rplT-pheMST-ihfA         
Operon arrangement:
Transcription unit        Promoter
ihfA
pheM
pheMST-ihfA
rplT
rplT-pheM
rpmI-rplT
infC-rpmI-rplT
infC
thrS-infC
thrS-infC-rpmI-rplT-pheMST-ihfA


Transcriptional Regulation      
Display Regulation             
Repressed by: NsrR, FNR


RNA cis-regulatory element    
Attenuation: Transcriptional
   
Cis-reg; leader Phenylalanine leader peptide
   
   


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2010 1798580 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2011 1798597 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2012 1799135 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2013 1799621 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Mayaux JF., Fayat G., Panvert M., Springer M., Grunberg-Manago M., Blanquet S., 1985, Control of phenylalanyl-tRNA synthetase genetic expression. Site-directed mutagenesis of the pheS, T operon regulatory region in vitro., J Mol Biol 184(1):31-44

 [2] Mayaux JF., Springer M., Graffe M., Fromant M., Fayat G., 1984, IS4 transposition in the attenuator region of the Escherichia coli pheS,T operon., Gene 30(1-3):137-46

 [3] Springer M., Graffe M., Mayaux JF., Dardel F., Fayat G., Blanquet S., Grunberg-Manago M., 1987, Open reading frames in the control regions of the phenylalanyl-tRNA synthetase operon of E. coli., Biochimie 69(10):1065-70

 [4] Springer M., Mayaux JF., Fayat G., Plumbridge JA., Graffe M., Blanquet S., Grunberg-Manago M., 1985, Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon., J Mol Biol 181(4):467-78

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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