RegulonDB

Gene
Name:	pheM
Synonym(s):	ECK1713, EG11272, b1715, phtL
Genome position(nucleotides):	1799226 <-- 1799270
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	35.56
External database links:

ASAP:	ABE-0005727
ECHOBASE:	EB1251
ECOLIHUB:	pheM
OU-MICROARRAY:	b1715
COLOMBOS:	pheM

Product

Name:

pheST-ihfA operon leader peptide

Synonym(s):

PheM, PhtL, phenylalanyl-tRNA synthetase (pheST) operon leader peptide

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

membrane

Molecular weight:

1.762

Isoelectric point:

9.355

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.3 - protein related --> 2.3.1 - amino acid -activation
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane
	biological_process	GO:0006355 - regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Mayaux JF., et al., 1985
[2] Mayaux JF., et al., 1984
[3] Springer M., et al., 1987
[4] Springer M., et al., 1985
[5] Svanberg Frisinger F., et al., 2021

External database links:

ECOCYC:

ECOLIWIKI:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

thrS-infC-rpmI-rplT-pheMST-ihfA

Operon arrangement:

Transcription unit	Promoter
ihfA	ihfAp4
pheM	pheMp
pheMST-ihfA	pheMp
rplT	rplTp
rplT-pheM	rplTp
rpmI-rplT	rpmIp
infC-rpmI-rplT	infCp2
infC	infCp1
thrS-infC	thrSp
thrS-infC-rpmI-rplT-pheMST-ihfA	thrSp

Transcriptional Regulation
	Display Regulation
Repressed by:	NsrR, FNR

RNA cis-regulatory element

Attenuation:	Transcriptional

Cis-reg; leader	Phenylalanine leader peptide

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2010	1798580	reverse	nd	[RS-EPT-CBR]	[6]
promoter	TSS_2011	1798597	reverse	nd	[RS-EPT-CBR]	[6]
promoter	TSS_2012	1799135	reverse	nd	[RS-EPT-CBR]	[6]
promoter	TSS_2013	1799621	reverse	nd	[RS-EPT-CBR]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Mayaux JF., Fayat G., Panvert M., Springer M., Grunberg-Manago M., Blanquet S., 1985, Control of phenylalanyl-tRNA synthetase genetic expression. Site-directed mutagenesis of the pheS, T operon regulatory region in vitro., J Mol Biol 184(1):31-44
[2] Mayaux JF., Springer M., Graffe M., Fromant M., Fayat G., 1984, IS4 transposition in the attenuator region of the Escherichia coli pheS,T operon., Gene 30(1-3):137-46
[3] Springer M., Graffe M., Mayaux JF., Dardel F., Fayat G., Blanquet S., Grunberg-Manago M., 1987, Open reading frames in the control regions of the phenylalanyl-tRNA synthetase operon of E. coli., Biochimie 69(10):1065-70
[4] Springer M., Mayaux JF., Fayat G., Plumbridge JA., Graffe M., Blanquet S., Grunberg-Manago M., 1985, Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon., J Mol Biol 181(4):467-78
[5] Svanberg Frisinger F., Jana B., Donadio S., Guardabassi L., 2021, In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli., Antibiotics (Basel) 10(6)
[6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.