RegulonDB RegulonDB 11.1: Gene Form
   

murA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

murA sfsB ibaG terminator TSS_3501 (cluster) TSS_3501 (cluster) TSS_3500 TSS_3500

Gene      
Name: murA    Texpresso search in the literature
Synonym(s): ECK3178, EG11358, b3189, mrbA, murZ
Genome position(nucleotides): 3335235 <-- 3336494
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.33
External database links:  
ASAP:
 ABE-0010479
CGSC:
 33518
ECHOBASE:
 EB1333
ECOLIHUB:
 murA
OU-MICROARRAY:
 b3189
STRING:
 511145.b3189
COLOMBOS: murA


Product      
Name: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Synonym(s): MrbA, MurA, MurZ
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 44.818
Isoelectric point: 6.071
Motif(s):
 
Type Positions Sequence Comment
6 -> 406 VQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNVPKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDKVSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGVERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVGEDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMHVPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRIYHIDRGYERIEDKL
22 -> 23 KN UniProt: Phosphoenolpyruvate binding; Sequence Annotation Type: region of interest.
120 -> 124 RPVDL UniProt: UDP-N-acetylglucosamine binding; Sequence Annotation Type: region of interest.
160 -> 163 KVSV UniProt: UDP-N-acetylglucosamine binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)
  6 - cell structure --> 6.2 - murein
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016765 - transferase activity, transferring alkyl or aryl (other than methyl) groups
GO:0016740 - transferase activity
GO:0008760 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity
biological_process GO:0051301 - cell division
GO:0007049 - cell cycle
GO:0009252 - peptidoglycan biosynthetic process
GO:0008360 - regulation of cell shape
GO:0019277 - UDP-N-acetylgalactosamine biosynthetic process
GO:0071555 - cell wall organization
Note(s): Note(s): ...[more].
Reference(s): [1] Nicolaou SA., et al., 2012
External database links:  
ALPHAFOLD:
 P0A749
DIP:
 DIP-48060N
ECOCYC:
 UDPNACETYLGLUCOSAMENOLPYRTRANS-MONOMER
ECOLIWIKI:
 b3189
INTERPRO:
 IPR013792
INTERPRO:
 IPR036968
INTERPRO:
 IPR005750
INTERPRO:
 IPR001986
MODBASE:
 P0A749
PDB:
 3ISS
PDB:
 2Z2C
PDB:
 3SWD
PDB:
 1A2N
PDB:
 1UAE
PDB:
 3KQJ
PDB:
 3KR6
PFAM:
 PF00275
PRIDE:
 P0A749
PRODB:
 PRO_000023312
REFSEQ:
 NP_417656
SMR:
 P0A749
UNIPROT:
 P0A749


Operon      
Name: ibaG-murA         
Operon arrangement:
Transcription unit        Promoter
ibaG-murA
ibaG
murA


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3500 3336517 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3501 (cluster) 3336555 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Nicolaou SA., Gaida SM., Papoutsakis ET., 2012, Exploring the combinatorial genomic space in Escherichia coli for ethanol tolerance., Biotechnol J 7(11):1337-45

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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