RegulonDB RegulonDB 10.9: Gene Form
   

rpiA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

serA argP rpiA yqfE ArgP PhoB ArgP CRP Nac Nac anti-anti-terminator anti-terminator terminator argPp argPp TSS_3206 TSS_3206 TSS_3205 (cluster) TSS_3205 (cluster) rpiAp rpiAp serAp2 serAp2 serAp1 serAp1 TSS_3203 TSS_3203 TSS_3202 (cluster) TSS_3202 (cluster) TSS_3201 TSS_3201 TSS_3200 TSS_3200 TSS_3199 TSS_3199 TSS_3198 TSS_3198 TSS_3197 TSS_3197 TSS_3196 TSS_3196 TSS_3195 TSS_3195 TSS_3194 TSS_3194 TSS_3193 TSS_3193

Gene      
Name: rpiA    Texpresso search in the literature
Synonym(s): ECK2910, EG11443, b2914, ygfC
Genome position(nucleotides): 3058666 <-- 3059325 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.82
External database links:  
ASAP:
ABE-0009567
CGSC:
264
ECHOBASE:
EB1413
ECOLIHUB:
rpiA
MIM:
608611
OU-MICROARRAY:
b2914
STRING:
511145.b2914
COLOMBOS: rpiA


Product      
Name: ribose-5-phosphate isomerase A
Synonym(s): RpiA, YgfC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 22.86
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence
28 -> 31 TGST
49 -> 213 AVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTREKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGVVTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDG
94 -> 94 K
81 -> 84 DGAD
84 -> 84 D

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.3 - non-oxidative branch, pentose pathway
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016853 - isomerase activity
GO:0004751 - ribose-5-phosphate isomerase activity
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
biological_process GO:0009052 - pentose-phosphate shunt, non-oxidative branch
GO:0006014 - D-ribose metabolic process
GO:0006098 - pentose-phosphate shunt
Note(s): Note(s): ...[more].
Reference(s): [1] Edwards JS., et al., 2000
[2] Wang Z., et al., 2006
External database links:  
DIP:
DIP-10739N
ECOCYC:
RIB5PISOMA-MONOMER
ECOLIWIKI:
b2914
INTERPRO:
IPR020672
INTERPRO:
IPR037171
INTERPRO:
IPR004788
MODBASE:
P0A7Z0
PANTHER:
PTHR11934
PDB:
1O8B
PDB:
1LKZ
PDB:
1KS2
PFAM:
PF06026
PRIDE:
P0A7Z0
PRODB:
PRO_000023806
REFSEQ:
NP_417389
SMR:
P0A7Z0
UNIPROT:
P0A7Z0


Operon      
Name: rpiA         
Operon arrangement:
Transcription unit        Promoter
rpiA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3193 3057340 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3194 3057467 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3195 3057593 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3196 3057726 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3197 3057734 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3198 3058242 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3199 3058303 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3200 3058379 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3201 3058419 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3202 (cluster) 3058434 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [3]
  promoter TSS_3203 3058455 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3205 (cluster) 3059719 forward For this promoter, there
Read more >
[RS-EPT-CBR] [3]
  promoter TSS_3206 3059729 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Edwards JS., Palsson BO., 2000, Robustness analysis of the Escherichia coli metabolic network., Biotechnol Prog 16(6):927-39

 [2] Wang Z., Xiang L., Shao J., Wegrzyn A., Wegrzyn G., 2006, Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism., Microb Cell Fact 5:34

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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