Gene
Name:	holE
Synonym(s):	ECK1843, EG11505, b1842
Genome position(nucleotides):	1925108 --> 1925338
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	48.48
External database links:

ASAP:	ABE-0006126
CGSC:	31439
ECHOBASE:	EB1468
ECOLIHUB:	holE
OU-MICROARRAY:	b1842
STRING:	511145.b1842
COLOMBOS:	holE

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	holEp5	1925020	forward	nd	[COMP-AINF]	[8]
promoter	holEp4	1925029	forward	nd	[COMP-AINF]	[8]
promoter	holEp6	1925086	forward	nd	[COMP-AINF]	[8]
promoter	TSS_2184	1925088	forward	nd	[RS-EPT-CBR]	[9]
promoter	yobBp8	1925209	forward	nd	[COMP-AINF]	[8]
promoter	TSS_2185	1925307	reverse	nd	[RS-EPT-CBR]	[9]
promoter	yobBp5	1925364	forward	nd	[COMP-AINF]	[8]
promoter	TSS_2186	1925389	forward	nd	[RS-EPT-CBR]	[9]
promoter	TSS_2187 (cluster)	1926096	forward	nd	[RS-EPT-CBR]	[9]

Evidence
[COMP-AINF] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] DeRose EF., Darden T., Harvey S., Gabel S., Perrino FW., Schaaper RM., London RE., 2003, Elucidation of the epsilon-theta subunit interface of Escherichia coli DNA polymerase III by NMR spectroscopy., Biochemistry 42(13):3635-44
[2] Gupta R., Hamdan SM., Dixon NE., Sheil MM., Beck JL., 2004, Application of electrospray ionization mass spectrometry to study the hydrophobic interaction between the epsilon and theta subunits of DNA polymerase III., Protein Sci 13(11):2878-87
[3] Jonczyk P., Nowicka A., Fijalkowska IJ., Schaaper RM., Ciesla Z., 1998, In vivo protein interactions within the Escherichia coli DNA polymerase III core., J Bacteriol 180(6):1563-6
[4] Kim DR., McHenry CS., 1996, In vivo assembly of overproduced DNA polymerase III. Overproduction, purification, and characterization of the alpha, alpha-epsilon, and alpha-epsilon-theta subunits., J Biol Chem 271(34):20681-9
[5] Li D., Allen DL., Harvey S., Perrino FW., Schaaper RM., London RE., 1999, A preliminary CD and NMR study of the Escherichia coli DNA polymerase III theta subunit., Proteins 36(1):111-6
[6] Ozawa K., Horan NP., Robinson A., Yagi H., Hill FR., Jergic S., Xu ZQ., Loscha KV., Li N., Tehei M., Oakley AJ., Otting G., Huber T., Dixon NE., 2013, Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, epsilon, θ and β reveals a highly flexible arrangement of the proofreading domain., Nucleic Acids Res 41(10):5354-67
[7] Ozawa K., Jergic S., Park AY., Dixon NE., Otting G., 2008, The proofreading exonuclease subunit epsilon of Escherichia coli DNA polymerase III is tethered to the polymerase subunit alpha via a flexible linker., Nucleic Acids Res 36(15):5074-82
[8] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[9] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.