RegulonDB RegulonDB 10.10: Gene Form
   

pyrH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tsf pyrH frr ppGpp terminator dxrp5 dxrp5 dxrp4 dxrp4 dxrp3 dxrp3 TSS_332 TSS_332 frrp6 frrp6 frrp frrp frrp3 frrp3 TSS_330 TSS_330 TSS_329 (cluster) TSS_329 (cluster) pyrHp pyrHp TSS_327 TSS_327 TSS_326 TSS_326

Gene      
Name: pyrH    Texpresso search in the literature
Synonym(s): ECK0170, EG11539, b0171, smbA, umk
Genome position(nucleotides): 191855 --> 192580 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.75
External database links:  
ASAP:
 ABE-0000582
CGSC:
 324
ECHOBASE:
 EB1501
ECOLIHUB:
 pyrH
OU-MICROARRAY:
 b0171
STRING:
 511145.b0171
COLOMBOS: pyrH


Product      
Name: UMP kinase
Synonym(s): PyrH, SmbA, Umk
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 25.97
Isoelectric point: 7.512
Motif(s):
 
Type Positions Sequence
77 -> 77 D
64 -> 69 AGLAKA
159 -> 159 D
15 -> 18 KLSG
23 -> 28 GTEGFG

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0009041 - uridylate kinase activity
GO:0042802 - identical protein binding
GO:0033862 - UMP kinase activity
biological_process GO:0046940 - nucleoside monophosphate phosphorylation
GO:0008152 - metabolic process
GO:0006221 - pyrimidine nucleotide biosynthetic process
GO:0016310 - phosphorylation
GO:0044210 - 'de novo' CTP biosynthetic process
GO:0006225 - UDP biosynthetic process
External database links:  
DIP:
 DIP-31830N
ECOCYC:
 UMPKI-MONOMER
ECOLIWIKI:
 b0171
INTERPRO:
 IPR001048
INTERPRO:
 IPR011817
INTERPRO:
 IPR015963
INTERPRO:
 IPR036393
MODBASE:
 P0A7E9
PDB:
 2V4Y
PDB:
 2BNF
PDB:
 2BNE
PDB:
 2BND
PFAM:
 PF00696
PRIDE:
 P0A7E9
PRODB:
 PRO_000023663
REFSEQ:
 NP_414713
SMR:
 P0A7E9
UNIPROT:
 P0A7E9


Operon      
Name: pyrH         
Operon arrangement:
Transcription unit        Promoter
pyrH


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_326 191752 forward nd [RS-EPT-CBR] [1]
  promoter TSS_327 191754 forward nd [RS-EPT-CBR] [1]
  promoter TSS_329 (cluster) 191819 forward nd [RS-EPT-CBR] [1]
  promoter TSS_330 192617 forward nd [RS-EPT-CBR] [1]
  promoter frrp3 192812 forward nd [ICWHO] [2]
  promoter frrp6 192849 forward nd [ICWHO] [2]
  promoter TSS_332 192941 forward nd [RS-EPT-CBR] [1]
  promoter dxrp3 193335 forward nd [ICWHO] [2]
  promoter dxrp4 193338 forward nd [ICWHO] [2]
  promoter dxrp5 193411 forward nd [ICWHO] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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