RegulonDB RegulonDB 11.1: Gene Form
   

kup gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

kup ravA rbsD viaA RbsR CRP FNR FNR terminator anti-terminator anti-anti-terminator rbsDp rbsDp kupp3 kupp3 TSS_4359 TSS_4359 kupp2 kupp2 TSS_4358 TSS_4358 TSS_4357 TSS_4357 ravAp ravAp kupp1 kupp1 TSS_4356 TSS_4356 TSS_4355 TSS_4355

Gene      
Name: kup    Texpresso search in the literature
Synonym(s): ECK3741, EG11541, b3747, trkD
Genome position(nucleotides): 3931316 --> 3933184
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.42
External database links:  
ASAP:
 ABE-0012251
CGSC:
 81
ECHOBASE:
 EB1503
ECOLIHUB:
 kup
OU-MICROARRAY:
 b3747
STRING:
 511145.b3747
COLOMBOS: kup


Product      
Name: K+:H+ symporter Kup
Synonym(s): Kup, TrkD
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 69.294
Isoelectric point: 9.282
Motif(s):
 
Type Positions Sequence Comment
9 -> 29 LPAITLAAIGVVYGDIGTSPL UniProt: Helical.
14 -> 544 LAAIGVVYGDIGTSPLYTLRECLSGQFGFGVERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSMLVIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRE
23 -> 23 D D → A: loss of function mutation
49 -> 69 VFGFLSLIFWLLIFVVSIKYL UniProt: Helical.
103 -> 123 VIMGLIGGSFFYGEVVITPAI UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015387 - potassium:proton symporter activity
GO:0015293 - symporter activity
GO:0015079 - potassium ion transmembrane transporter activity
biological_process GO:0071278 - cellular response to cesium ion
GO:0006811 - ion transport
GO:0006813 - potassium ion transport
GO:0071805 - potassium ion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Dosch DC., et al., 1991
External database links:  
ALPHAFOLD:
 P63183
ECOCYC:
 KUP-MONOMER
ECOLIWIKI:
 b3747
INTERPRO:
 IPR003855
INTERPRO:
 IPR023051
PANTHER:
 PTHR30540
PFAM:
 PF02705
PRIDE:
 P63183
PRODB:
 PRO_000023068
REFSEQ:
 YP_026244
UNIPROT:
 P63183


Operon      
Name: kup         
Operon arrangement:
Transcription unit        Promoter
kup
kup
kup


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4355 3931076 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4356 3931080 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4357 3931130 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4358 3931158 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4359 3931257 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Dosch DC., Helmer GL., Sutton SH., Salvacion FF., Epstein W., 1991, Genetic analysis of potassium transport loci in Escherichia coli: evidence for three constitutive systems mediating uptake potassium., J Bacteriol 173(2):687-96

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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