RegulonDB RegulonDB 10.10: Gene Form
   

eptA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

eptA adiC basS basR pmrR basSp4 basSp4 TSS_4921 TSS_4921 TSS_4920 TSS_4920 TSS_4919 TSS_4919

Gene      
Name: eptA    Texpresso search in the literature
Synonym(s): ECK4107, EG11613, b4114, yjdB
Genome position(nucleotides): 4333947 <-- 4335590 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.45
External database links:  
ASAP:
 ABE-0013469
ECHOBASE:
 EB1570
ECOLIHUB:
 eptA
OU-MICROARRAY:
 b4114
STRING:
 511145.b4114
COLOMBOS: eptA


Product      
Name: phosphoethanolamine transferase EptA
Synonym(s): EptA, YjdB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 61.667
Isoelectric point: 6.825
Motif(s):
 
Type Positions Sequence
238 -> 527 TILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGV
80 -> 100 CLFILVGAAAQYFIMTYGIVI
10 -> 30 SLNLLAWLLLAAFYISICLNI
124 -> 144 MLLTLGFSGVLAALIACWIKI
155 -> 175 LFRGANILVSVLLILLVAALF

 
Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0016772 - transferase activity, transferring phosphorus-containing groups
GO:0016776 - phosphotransferase activity, phosphate group as acceptor
GO:0008484 - sulfuric ester hydrolase activity
biological_process GO:0006629 - lipid metabolic process
GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009244 - lipopolysaccharide core region biosynthetic process
GO:0009245 - lipid A biosynthetic process
GO:0046677 - response to antibiotic
Note(s): Note(s): ...[more].
Reference(s): [1] Klein G., et al., 2013
[2] Raetz CR., et al., 2007
External database links:  
DIP:
 DIP-12561N
ECOCYC:
 EG11613-MONOMER
ECOLIWIKI:
 b4114
INTERPRO:
 IPR040423
INTERPRO:
 IPR017850
INTERPRO:
 IPR012549
INTERPRO:
 IPR000917
MODBASE:
 P30845
PANTHER:
 PTHR30443
PFAM:
 PF00884
PFAM:
 PF08019
PRIDE:
 P30845
PRODB:
 PRO_000022529
REFSEQ:
 NP_418538
SMR:
 P30845
UNIPROT:
 P30845


Operon      
Name: eptA         
Operon arrangement:
Transcription unit        Promoter
eptA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4919 4332618 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4920 4332748 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4921 4332753 reverse nd [RS-EPT-CBR] [3]
  promoter basSp4 4333477 reverse nd [ICWHO] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Klein G., Muller-Loennies S., Lindner B., Kobylak N., Brade H., Raina S., 2013, Molecular and structural basis of inner core lipopolysaccharide alterations in Escherichia coli: incorporation of glucuronic acid and phosphoethanolamine in the heptose region., J Biol Chem 288(12):8111-27

 [2] Raetz CR., Reynolds CM., Trent MS., Bishop RE., 2007, Lipid A modification systems in gram-negative bacteria., Annu Rev Biochem 76:295-329

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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