RegulonDB RegulonDB 10.9: Gene Form
   

psuK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fruA psuT psuK psuG TSS_2462 TSS_2462 psuGp4 psuGp4 psuTp2 psuTp2

Gene      
Name: psuK    Texpresso search in the literature
Synonym(s): ECK2159, EG11646, b2166, pscK, yeiC
Genome position(nucleotides): 2258355 <-- 2259296 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 47.03
External database links:  
ASAP:
 ABE-0007168
ECHOBASE:
 EB1599
ECOLIHUB:
 pscK
OU-MICROARRAY:
 b2166
STRING:
 511145.b2166
COLOMBOS: psuK


Product      
Name: putative pseudouridine kinase
Synonym(s): PscK, PsuK, YeiC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.573
Isoelectric point: 4.507
Motif(s):
 
Type Positions Sequence
5 -> 295 DYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEYTNNP

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.2 - pyrimidine biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0016773 - phosphotransferase activity, alcohol group as acceptor
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0050225 - pseudouridine kinase activity
biological_process GO:0016310 - phosphorylation
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
 EG11646-MONOMER
ECOLIWIKI:
 b2166
INTERPRO:
 IPR002173
INTERPRO:
 IPR011611
INTERPRO:
 IPR029056
MODBASE:
 P30235
PFAM:
 PF00294
PRIDE:
 P30235
PRODB:
 PRO_000023626
PROSITE:
 PS00583
PROSITE:
 PS00584
REFSEQ:
 NP_416671
SMR:
 P30235
UNIPROT:
 P30235


Operon      
Name: psuKG         
Operon arrangement:
Transcription unit        Promoter
psuKG


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter psuTp2 2257496 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter psuGp4 2258557 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter TSS_2462 2260442 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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